Kinky microtubules: bending and breaking induced by fixation in vitro with glutaraldehyde and formaldehyde.
Kinky microtubules: bending and breaking induced by fixation in vitro with glutaraldehyde and formaldehyde.
复制标题
扭结微管：戊二醛和甲醛体外固定引起的弯曲和断裂。
DOI：
10.1002/cm.970200403
复制
发表时间：
1991
影响因子：
--
通讯作者：
WilliamsJr,RC
中科院分区：
文献类型：
--
作者：
Cross,AR;WilliamsJr,RC
我们使用视频增强光学显微镜来研究微管的整体形状的变化，这些微管是由通常用于体外研究的醛固定方法产生的。这些方法带来的变化包括变形和破坏。影响的严重程度取决于所使用的固定剂，并随着其浓度和固定时间的增加而增加。这些变化是在各种条件下观察到的，例如短暂暴露在3.7%的甲醛中，或稍微长时间暴露在浓度低至0.05%的戊二醛中。观察到的扭曲解释了为什么微管在电子显微镜中通常表现为弯曲或弯曲，而在视频增强光学显微镜中则表现为相对刚性和线形。观察到的断裂意味着，在从醛固定的微管的测量中推断长度分布时必须谨慎。
We have employed video‐enhanced light microscopy to study alterations of the overall shape of microtubules that are produced by the aldehyde fixation methods commonly employed to study them in vitro. Changes brought about by these methods include deformation and breakage. The severity of the effects depends on the fixative employed and increases with its concentration, and with the time of fixation. The changes are observed under a variety of conditions, such as brief exposure to 3.7% formaldehyde, or somewhat longer exposure to glutaraldehyde at concentrations as low as 0.05%. The observed distortion explains why microtubules usually appear curved or sinuous in electron micrographs while appearing relatively rigid and linear in video‐enhanced light microscopy. The observed breakage implies that caution must be used in inferring length distributions from measurements of aldehyde‐fixed microtubules.