Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis.
Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis.
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DOI：
10.1016/j.ydbio.2010.06.028
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发表时间：
2010-09-15
影响因子：
2.7
通讯作者：
Kiehart DP
中科院分区：
文献类型：
--
作者：
Franke JD;Montague RA;Kiehart DP
后生动物的发育涉及无数需要细胞骨架功能的动态细胞过程。非肌肉肌球蛋白II在胚胎发育中起着至关重要的作用；然而，关于它在胚胎后发育中的作用的知识，甚至在果蝇等模式生物中的作用，直到最近才被揭示。在这项研究中，产生了截断等位基因，并以分级的方式使非肌肉肌球蛋白II功能的条件扰动成为可能。在翅膀发育过程中，它们展示了非肌肉肌球蛋白II的新作用，包括在背侧和腹侧翅膀上皮片之间的粘连；在每个细胞的远端顶端形成单一的基于肌动蛋白的翼毛；在形成无分支的翼毛；以及在静脉和交叉静脉的正确定位中。这些表型中的许多与在机翼中使用功能等位基因丢失进行克隆镶嵌分析时观察到的表型重叠。对非肌肉肌球蛋白II的额外要求是其他基于肌动蛋白的细胞突起(微毛和大毛)的正确形成。我们证实并扩展了遗传相互作用研究，以表明非肌肉肌球蛋白II和非传统肌球蛋白，由皱缩(CK/MyoVIIA)编码，在翅膀发育所需的多个过程中起拮抗作用。最后，我们证明了截断等位基因可以通过两种不同的机制扰乱非肌肉肌球蛋白II的功能--通过滴定内源性重链上的轻链或通过招募内源性重链进入细胞内聚集体。通过允许以受控方式干扰肌球蛋白II的功能，这些新工具能够阐明非肌肉肌球蛋白II在果蝇目标发育阶段的胚胎后角色。
Metazoan development involves a myriad of dynamic cellular processes that require cytoskeletal function. Nonmuscle myosin II plays essential roles in embryonic development; however, knowledge of its role in post-embryonic development, even in model organisms such as Drosophila melanogaster, is only recently being revealed. In this study, truncation alleles were generated and enable the conditional perturbation, in a graded fashion, of nonmuscle myosin II function. During wing development they demonstrate novel roles for nonmuscle myosin II, including in adhesion between the dorsal and ventral wing epithelial sheets; in the formation of a single actin-based wing hair from the distal vertex of each cell; in forming unbranched wing hairs; and in the correct positioning of veins and crossveins. Many of these phenotypes overlap with those observed when clonal mosaic analysis was performed in the wing using loss of function alleles. Additional requirements for nonmuscle myosin II are in the correct formation of other actin-based cellular protrusions (microchaetae and macrochaetae). We confirm and extend genetic interaction studies to show that nonmuscle myosin II and an unconventional myosin, encoded by crinkled (ck/MyoVIIA), act antagonistically in multiple processes necessary for wing development. Lastly, we demonstrate that truncation alleles can perturb nonmuscle myosin II function via two distinct mechanisms – by titrating light chains away from endogenous heavy chains or by recruiting endogenous heavy chains into intracellular aggregates. By allowing myosin II function to be perturbed in a controlled manner, these novel tools enable the elucidation of post-embryonic roles for nonmuscle myosin II during targeted stages of fly development.
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影响因子：
2.7
作者：
Bloor, JW;Kiehart, DP
通讯作者：
Kiehart, DP
影响因子：
9.2
作者：
Franke, JD;Montague, RA;Kiehart, DP
通讯作者：
Kiehart, DP
影响因子：
21.3
作者：
Choi, Colin K.;Vicente-Manzanares, Miguel;Zareno, Jessica;Whitmore, Leanna A.;Mogilner, Alex;Horwitz, Alan Rick
通讯作者：
Horwitz, Alan Rick
影响因子：
4.8
作者：
Conti, MA;Even-Ram, S;Adelstein, RS
通讯作者：
Adelstein, RS
影响因子：
11.4
作者：
HARRISON, DA;BINARI, R;PERRIMON, N
通讯作者：
PERRIMON, N