High-Order Assembly toward Polysaccharide-Based Complex Coacervate Nanodroplets Capable of Targeting Cancer Cells
High-Order Assembly toward Polysaccharide-Based Complex Coacervate Nanodroplets Capable of Targeting Cancer Cells
复制标题
针对能够靶向癌细胞的基于多糖的复合凝聚纳米液滴的高阶组装。
DOI：
10.1021/acs.langmuir.0c01458
复制
发表时间：
2020-07-28
期刊：
影响因子：
3.9
通讯作者：
Dou, Hongjing
中科院分区：
文献类型：
--
作者：
Liu, Lingshan;Xu, Yuan;Dou, Hongjing
高阶组装在包含大量生物大分子的活生物体的形成中起着重要作用，因此，启发了可以以高效率负载大分子有效载荷的纳米材料的构建。在此，通过选择阴离子透明质酸（HA）作为模型负载，我们证明了如何静电相互作用诱导的高阶组装可以用来有效地加载到复杂的凝聚纳米液滴的生物大分子。所得到的组件主要由HA和阳离子壳寡糖/葡聚糖（COS/Dex）纳米凝胶组成，具有可控的结构，同时也表现出生物功能。组装体中的HA能够通过本文阐明的CD 44介导的内吞途径靶向CD 44过表达的肿瘤细胞。因此，本研究提供了一种可靠的方法，通过静电吸引诱导的高阶组装的大分子有效载荷到复杂的凝聚纳米液滴的有效负载。
High-order assembly plays a significant role in the formation of living organisms containing a large number of biomacromolecules and, thus, enlightens the construction of nanomaterials that can load macromolecular payloads at a high efficiency. Herein, by choosing anionic hyaluronic acid (HA) as a model payload, we demonstrated how the electrostatic-interaction-induced high-order assembly can be used to load efficiently biomacromolecules into complex coacervate nanodroplets. The resultant assemblies were primarily composed of HA and cationic chitosan oligosaccharide/dextran (COS/Dex) nanogels and had a controllable structure while also exhibiting biological functionality. HA in the assemblies is capable of targeting CD44-overexpressed tumor cells through CD44-mediated endocytic pathways, which are elucidated herein. Therefore, this study provides a reliable approach for the efficient loading of macromolecular payloads into complex coacervate nanodroplets via electrostatic-attraction-induced high-order assembly.