Zebrafish Craniofacial Development: A Window into Early Patterning.
Zebrafish Craniofacial Development: A Window into Early Patterning.
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DOI：
10.1016/bs.ctdb.2015.07.001
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发表时间：
2015
影响因子：
--
通讯作者：
Crump G
中科院分区：
文献类型：
--
作者：
Mork L;Crump G
面部和颅骨的形成涉及一系列复杂的发育事件，这些事件由来自神经嵴、内胚层、中胚层和外胚层的细胞介导。虽然脊椎动物拥有巨大的多样性的成人面部形态，基本的信号通路和细胞事件，塑造胚胎中的新生颅面骨骼已被证明是高度保守的从鱼到人。斑马鱼Danio rerio，一个小的淡水鲤科鱼类从印度东部，一直作为颅面发育的流行模型自20世纪90年代以来。斑马鱼模型的独特优势包括在幼虫阶段简化骨骼，获得快速发育的胚胎进行实时成像，以及对转基因和复杂遗传学的顺从性。在这一章中，我们描述了斑马鱼颅面骨骼的解剖学;它作为哺乳动物颌骨、中耳、腭和颅缝模型的应用;鱼类中的上级成像技术为面部形态发生的动力学提供了前所未有的见解;利用斑马鱼来破译颅面生物学的遗传基础;最后，我们将对斑马鱼颅面研究的最有前途的未来应用进行一次展望。
The formation of the face and skull involves a complex series of developmental events mediated by cells derived from the neural crest, endoderm, mesoderm, and ectoderm. Although vertebrates boast an enormous diversity of adult facial morphologies, the fundamental signaling pathways and cellular events that sculpt the nascent craniofacial skeleton in the embryo have proven to be highly conserved from fish to man. The zebrafish Danio rerio, a small freshwater cyprinid fish from eastern India, has served as a popular model of craniofacial development since the 1990s. Unique strengths of the zebrafish model include a simplified skeleton during larval stages, access to rapidly developing embryos for live imaging, and amenability to transgenesis and complex genetics. In this chapter, we describe the anatomy of the zebrafish craniofacial skeleton; its applications as models for the mammalian jaw, middle ear, palate, and cranial sutures; the superior imaging technology available in fish that has provided unprecedented insights into the dynamics of facial morphogenesis; the use of the zebrafish to decipher the genetic underpinnings of craniofacial biology; and finally a glimpse into the most promising future applications of zebrafish craniofacial research.