EIT-Inspired Microfluidic Cytometer for Single-Cell Dielectric Spectroscopy
EIT-Inspired Microfluidic Cytometer for Single-Cell Dielectric Spectroscopy
复制标题
DOI：
10.1109/jmems.2010.2067204
复制
发表时间：
2010-10-01
影响因子：
2.7
通讯作者：
Maceri, Franco
中科院分区：
文献类型：
--
作者：
Caselli, Federica;Bisegna, Paolo;Maceri, Franco
本文提出了一种新的用于单细胞介电谱的微流控细胞仪，并通过有限元模型进行了分析。该设备受电阻抗断层扫描的启发，包括两个环形电极阵列，而不是仅仅两对共面或平行面对的电极，从而允许刺激和测量模式的极大通用性。特别地，使用具有不同空间取向的刺激模式提供了除了定量细胞体积估计之外的关于细胞形态的信息。此外，由于电极极化在低频下的性能限制被克服，由于一个独特的记录方案：电流被注入电极对之间，并在剩余的电极使用高输入阻抗差分放大器测量所得的电压。这些特征显著增强了细胞仪的辨别能力。
A new microfluidic cytometer for single-cell dielectric spectroscopy is proposed in this paper and analyzed in silico by means of a finite-element model. The device, inspired by electrical impedance tomography, includes two circumferential arrays of electrodes instead of just two pairs of coplanar or parallel-facing electrodes, thus allowing a great versatility in stimulation and measurement patterns. In particular, using stimulation patterns with different spatial orientation provides information on cell morphology, besides quantitative cell-volume estimation. Moreover, the performance limitation at low frequency due to electrode polarization is overcome, owing to a peculiar recording scheme: Current is injected between an electrode pair, and the resulting voltages are measured at remaining electrodes using high-input-impedance differential amplifiers. These features significantly enhance the cytometer discrimination capabilities.