Effects of membrane lipid composition and antibacterial drugs on the rigidity of Escherichia coli: Different contributions of various bacterial substructures
Effects of membrane lipid composition and antibacterial drugs on the rigidity of Escherichia coli: Different contributions of various bacterial substructures
复制标题
膜脂成分和抗菌药物对大肠杆菌刚性的影响：不同细菌亚结构的不同贡献
DOI：
10.1002/sca.21243
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Li, Ming;Gan, Chaoye;Chen, Yong
刚性/硬度是细菌的一项重要生物力学特性，且可能与多种细菌活动相关。虽然对细菌细胞膜的刚性或流动性已有广泛研究，但不同细菌亚结构对细菌刚性的贡献却较少有人探究。在此，我们选用了四种具有不同膜脂成分的大肠杆菌菌株，以及三种抗菌药物（乙二胺四乙酸（EDTA）、溶菌酶和链霉素）来特异性改变细菌亚结构。通过使用原子力显微镜（AFM），我们发现，磷脂酰乙醇胺（PE）缺陷型大肠杆菌菌株的平均高度和杨氏模量大于PE 阳性菌株，并且EDTA、EDTA 加溶菌酶（而非单独使用溶菌酶）以及链霉素，均导致这四种大肠杆菌菌株的高度和杨氏模量显著降低。我们的数据表明，膜脂成分、完整的外膜、细胞壁以及细胞质内容物均对细菌刚性有影响，但程度各异。《扫描》2016年第38卷第70 - 79页。（c）2015 威利期刊公司 。
The rigidity/stiffness is an important biomechanical property of bacteria and potentially correlated with many bacterial activities. While the rigidity or fluidity of the bacterial membrane has been extensively studied, the contributions of different bacterial substructures to the bacterial rigidity are less investigated. Here, we utilized four Escherichia coli (E. coli) strains with different membrane lipid compositions and three antibacterial drugs (EDTA, lysozyme, and streptomycin) to specifically alter bacterial substructures. By using atomic force microscopy (AFM), we found that the average height and Young's modulus of phosphatidylethanolamine (PE)-deficient E. coli strains were larger than those of PE+ strains and that EDTA, EDTA plus lysozyme instead of lysozyme alone, and streptomycin all caused significant decreases in height and Young's modulus of the four E. coli strains. Our data imply that membrane lipid composition, the integrated outer membrane, the cell wall, and the cytoplasmic content are all responsible for bacterial rigidity but to different extents. SCANNING 38:70-79, 2016. (c) 2015 Wiley Periodicals, Inc.