New insight into comprehensive analysis of INDETERMINATE DOMAIN (IDD) gene family in rice
New insight into comprehensive analysis of INDETERMINATE DOMAIN (IDD) gene family in rice
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水稻不确定域（IDD）基因家族综合分析的新见解
DOI：
10.1016/j.plaphy.2020.06.032
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发表时间：
2020
影响因子：
6.5
通讯作者：
Yu Zhao
中科院分区：
文献类型：
--
作者：
Ting Zhang;Mingfang Tan;Leping Geng;Jiajia Li;Yimeng Xiang;Bang Zhang;Yu Zhao
IDD转录因子（inflammatedomain，TF）是植物特异性锌指蛋白家族，在植物的发育过程和非生物胁迫中起着重要的调控作用，在其他植物中已发现IDD基因，但水稻IDD家族基因尚未在全基因组范围内进行研究。本研究在水稻基因组中鉴定了15个OsIDD基因，并将其分为两大类。对OsIDD蛋白进行保守基序分析和潜在相互作用蛋白分析。对OsIDD基因的外显子-内含子结构、顺式作用元件和表达谱进行了分析。外显子-内含子结构分析表明，OsIDD基因虽然含有不同数量的内含子，但总体结构相对保守;顺式作用元件分析表明，OsIDD基因大部分转录产物都能被各种非生物胁迫和植物激素诱导。通过qRT-PCR检测了低温和干旱条件下OsIDD基因的表达情况，以及外源生长素（2，4-D）、赤霉素（GA 3）和脱落酸（阿坝）处理后OsIDD基因的表达情况。这些结果表明OsIDDs可能在非生物胁迫和激素响应中发挥重要作用。OsIDDs在水稻不同组织器官中的表达差异表明OsIDDs可能参与水稻不同的发育过程。更有趣的是，蛋白质-蛋白质相互作用（PPI）的预测显示OsIDDs可以与一些组蛋白修饰剂合作。这些结果为进一步阐明OsIDD基因的分子机制和深入了解OsIDD基因在水稻中的生物学功能奠定了基础。
The INDETERMINATE DOMAIN (IDD) transcription factor (TF), as a family of plant-specific zinc-finger proteins, regulates a variety of development processes and abiotic stresses in plants.IDDgenes have been identified and characterized in other plants, however, the riceIDDfamily genes have not been investigated at genome-wide. In this study, 15OsIDDgenes were identified in rice genome and phylogenetically classified into two groups. Conserved motifs and potential interaction protein analysis about OsIDD proteins were carried out. Exon-intron structures,cis-acting elements and expression profiles ofOsIDDgenes were also examined. Exon-intron structures analysis revealed that overall structures ofOsIDDgenes were relatively conserved although they contained different numbers of introns.Cis-acting elements analysis suggested that mostOsIDDgene transcripts could be induced by various abiotic stresses and phytohormones. The expression patterns ofOsIDDgenes were detected by qRT-PCR under cold and drought conditions, and by exogenous auxin (2,4-D), gibberellin (GA3), and abscisic acid (ABA) treatments, respectively. The results showed that theOsIDDs might play essential roles under abiotic stresses and hormone responses. Distinct expression profiles in tissues/organs suggested thatOsIDDs might be involved in different development processes in rice. More interestingly, the prediction of protein-protein interactions (PPIs) revealed OsIDDs could cooperate with some histone modifiers. Yeast two-hybrid assays were performed and confirmed it. Collectively, these results provide a foundation for further elucidation on the molecular mechanisms ofOsIDDgenes and advance our understanding of their biological function in rice.