An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation
An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation
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DOI：
10.7554/elife.18853
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发表时间：
2016-11-16
期刊：
影响因子：
7.7
通讯作者：
Neupert, Walter
中科院分区：
文献类型：
--
作者：
Harner, Max E.;Unger, Ann-Katrin;Neupert, Walter
线粒体的代谢功能和结构密切相关。60多年前，嵴被发现是线粒体的特征性元素，含有氧化磷酸化的蛋白质复合物，但嵴是如何形成的，仍然是一个悬而未决的问题。在这里，我们提出的实验结果与酵母，支持一种新的假设存在两种分子途径，导致产生的层状和管状嵴。板层嵴的形成依赖于线粒体融合机制，通过线粒体和线粒体DNA稳态所需的途径。管状嵴是通过内界膜的内陷形成的，内界膜的内陷途径独立于融合机制。FiFo-ATP合酶的二聚化和MICOS复合物的存在是两种途径所必需的。建议提出的假设也适用于高等真核生物，因为在整个进化过程中，关键成分在结构和功能上是保守的。
Metabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present experimental results obtained with yeast that support a novel hypothesis on the existence of two molecular pathways that lead to the generation of lamellar and tubular cristae. Formation of lamellar cristae depends on the mitochondrial fusion machinery through a pathway that is required also for homeostasis of mitochondria and mitochondrial DNA. Tubular cristae are formed via invaginations of the inner boundary membrane by a pathway independent of the fusion machinery. Dimerization of the FiFo-ATP synthase and the presence of the MICOS complex are necessary for both pathways. The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution.