Retinoic acid has the potential to suppress endometriosis development.
Retinoic acid has the potential to suppress endometriosis development.
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DOI：
10.1186/s13048-015-0179-6
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发表时间：
2015-07-31
影响因子：
4
通讯作者：
Sugino N
中科院分区：
文献类型：
--
作者：
Yamagata Y;Takaki E;Shinagawa M;Okada M;Jozaki K;Lee L;Sato S;Maekawa R;Taketani T;Asada H;Tamura H;Nakai A;Sugino N
子宫内膜异位症是育龄期女性常见的雌激素依赖性疾病，其发病机制尚未完全阐明。视黄酸作为生物调节剂在细胞中具有多种功能，异常的类视黄酸代谢似乎与子宫内膜异位症的病变有关。为了评估全反式维甲酸（ATRA）在治疗中的潜力，在培养的子宫内膜异位症细胞和组织中进行了转录组分析和雌二醇测量。研究了经atra处理的卵巢子宫内膜异位基质细胞（ESC） mRNA的表达水平。还研究了OEC组织中雌二醇的产生。在添加ATRA的分离ESC培养中，提取总RNA，然后使用GeneChip进行转录组分析。经ATRA处理后，49个基因上调，4个基因下调。许多上调的基因与细胞增殖的负调控有关。此外，ATRA处理降低了将雌二醇转化为雌酮的17- β -脱氢酶2 (HSD17B2) mRNA的表达，并呈剂量依赖性，ELISA检测表明ATRA处理抑制了OEC组织中雌二醇的产生。视黄酸有抑制子宫内膜异位症发展的潜力。
Despite endometriosis is common estrogen dependent disease afflicting women in reproductive age, the pathogenesis has not been fully elucidated. Retinoic acid has various functions in cells as biologic modulator, and aberrant retinoid metabolism seems to be involved in the lesions of endometriosis. In order to evaluate the potential of all-trans retinoic acid (ATRA) for therapeutic treatment, a transcriptome analysis and estradiol measurements in cultured endometriotic cells and tissues were conducted. The mRNA expression levels in ATRA-treated endometriotic stromal cells (ESC) isolated from ovarian endometrial cysts (OEC) were investigated. Estradiol production in OEC tissues was also investigated. In the isolated ESC culture supplemented with ATRA for four days, total RNA was extracted followed by a transcriptome analysis using GeneChip. Forty-nine genes were upregulated and four genes were down-regulated by the ATRA treatment. Many upregulated genes were associated with the negative regulation of cellular proliferation. In addition, ATRA treatment decreased the mRNA expression of 17-beta-dehydrogenase 2 (HSD17B2) which converts estradiol into estrone in a dose-dependent manner, and the ELISA measurements indicated that estradiol production in the OEC tissue was inhibited by ATRA treatment. Retinoic acid has the potential to suppress endometriosis development.