SEQUENCE OF 6S RNA OF E-COLI
SEQUENCE OF 6S RNA OF E-COLI
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DOI：
10.1038/newbio229147a0
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发表时间：
1971-01-01
期刊：
影响因子：
--
通讯作者：
BROWNLEE, GG
中科院分区：
文献类型：
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作者：
BROWNLEE, GG
32 P标记的RNA测序的主要技术困难是部分酶解产物中复杂混合物的分离。我们最近改进了分离大于十核苷酸的寡核苷酸的方法，采用DEAE-纤维素薄层层析结合pH 3.5的醋酸纤维素离子电泳（参考文献1）。我们用这种技术测定了大肠杆菌6S RNA的核苷酸序列。
THE principal technical difficulty in sequencing uniformly32P-labelled RNA is the fractionation of the complicated mixture of products present in partial enzyme digests. We have recently improved the methods for the separation of oligonucleotides larger than decanucleotides by using thin-layer chromatography on DEAE-cellulose in conjunction with ionophoresis on cellulose acetate atpH 3.5 (ref. 1). We have used this technique to establish the nucleotide sequence of the 6S RNA ofEscherichia coli.