Granulimonas faecalis gen. nov., sp. nov., and Leptogranulimonas caecicola gen. nov., sp. nov., novel lactate-producing Atopobiaceae bacteria isolated from mouse intestines, and an emended description of the family Atopobiaceae
Granulimonas faecalis gen. nov., sp. nov., and Leptogranulimonas caecicola gen. nov., sp. nov., novel lactate-producing Atopobiaceae bacteria isolated from mouse intestines, and an emended description of the family Atopobiaceae
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粪颗粒单胞菌属
DOI：
10.1099/ijsem.0.005596
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发表时间：
2022
影响因子：
2.8
通讯作者：
Tamaki Hideyuki
中科院分区：
文献类型：
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作者：
Morinaga Kana;Kusada Hiroyuki;Sakamoto Sachiko;Murakami Takumi;Toyoda Atsushi;Mori Hiroshi;Meng Xian-Ying;Takashino Motoko;Murotomi Kazutoshi;Tamaki Hideyuki
从小鼠肠道中分离出两种严格厌氧、革兰氏染色阳性、非运动细菌（菌株 OPF53T 和 TOC12T）。菌株 OPF53T 和 TOC12T 分别在 pH 5.5-9.0 和 5.0-9.0 以及 30-45°C 的温度下生长。这些菌株的细胞形态分别为短棒状和棒状，细胞内有颗粒。 OPF53T 的主要细胞脂肪酸为 C18 : 1cis9 和 C18 : 1cis9 二甲基乙缩醛，而 TOC12T 的主要细胞脂肪酸为 C18 : 0 和 C18 : 1cis9。在OPF53T中，改性蛋白胨-酵母提取物-葡萄糖（PYG）发酵的主要终产物是乳酸、甲酸和丁酸，而除了这些酸之外，TOC12T还产生氢气。 OPF53T和TOC12T的基因组大小分别为2.2和2.0 Mbp，DNA G+C含量分别为69.1%和58.7%。 OPF53T和TOC12T的16S rRNA基因序列与Atopobiaceae科成员Olsenella phocaeensisMarseille-P2936T(94.3 %)和Olsenella umbonataKCTC 15140T(93.2 %)的相似性最高。系统发育分析表明，这两种分离株均与Atopobiaceae 科的其他属形成了不同的谱系。此外，这两个菌株的16S rRNA基因序列相似性相对较低（93.4%），可以通过其独特的性状（包括细胞形状、DNA G+C含量和主要脂肪酸谱）来区分。根据其多相分类学特性，这些分离株代表了 Atopobiaceae 科中两个新属的两个 noel 物种，其名称为粪粒单胞菌 (Granulimonas faecalisgen)。十一月，SP。十一月(OPF53T=JCM 35015T=KCTC 25474T) 和细粒单胞菌。十一月，SP。十一月建议（TOC12T=JCM 35017T=KCTC 25472T）。
Two strictly anaerobic, Gram-stain-positive, non-motile bacteria (strains OPF53Tand TOC12T) were isolated from mouse intestines. Strains OPF53Tand TOC12Tgrew at pH 5.5–9.0 and 5.0–9.0, respectively, and at temperatures of 30–45 °C. The cell morphologies of these strains were short rods and rods, respectively, and the cells possessed intracellular granules. The major cellular fatty acids of OPF53Twere C18 : 1cis9 and C18 : 1cis9 dimethyl acetal, whereas those of TOC12Twere C18 : 0and C18 : 1cis9. In OPF53T, the main end-products of modified peptone–yeast extract–glucose (PYG) fermentation were lactate, formate and butyrate, whereas, in addition to these acids, TOC12Talso produced hydrogen. The genomes of OPF53Tand TOC12Twere respectively 2.2 and 2.0 Mbp in size with a DNA G+C contents of 69.1 and 58.7 %. The 16S rRNA gene sequences of OPF53Tand TOC12Tshowed the highest similarity to members of the familyAtopobiaceae, namely,Olsenella phocaeensisMarseille-P2936T(94.3 %) andOlsenella umbonataKCTC 15140T(93.2 %), respectively. Phylogenetic analyses revealed that both isolates formed distinct lineages from other genera of the familyAtopobiaceae. In addition, the two strains were characterized by relatively low 16S rRNA gene sequence similarity (93.4 %) and can be distinguished by their distinctive traits (including cell shape, DNA G+C content, and major fatty acids profiles). On the basis of their polyphasic taxonomic properties, these isolates represent two noel species of two novel genera within the familyAtopobiaceae, for which the namesGranulimonas faecalisgen. nov., sp. nov. (OPF53T=JCM 35015T=KCTC 25474T) andLeptogranulimonas caecicolagen. nov., sp. nov. (TOC12T=JCM 35017T=KCTC 25472T) are proposed.