Food deprivation and the facilitatory effects of estrogen in female hamsters: the LH surge and locomotor activity.
Food deprivation and the facilitatory effects of estrogen in female hamsters: the LH surge and locomotor activity.
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雌性仓鼠的食物匮乏和雌激素的促进作用：LH 激增和运动活动。
DOI：
10.1016/0031-9384(96)00133-3
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发表时间：
1996
影响因子：
2.9
通讯作者：
Wade,GN
中科院分区：
文献类型：
--
作者：
Mangels,RA;Jetton,AE;Powers,JB;Wade,GN
两个实验研究了短期食物剥夺对雌激素影响的神经内分泌过程的影响。这些研究是由先前的工作提示，表明食物剥夺增加了免疫细胞化学鉴定的细胞中含有雌二醇受体的内侧视前区的卵巢切除雌性仓鼠。据推测，这是代谢燃料可用性的变化可能改变一个或多个系统对雌二醇的反应的一种方式。本研究的目的是研究雌二醇的两种作用，可能会受到食物剥夺的影响;这些是1）雌二醇对促黄体生成素（LH）激增的正反馈作用，和2）雌二醇对自发活动的促进作用。在实验1中，卵巢切除仓鼠给予雌二醇，之前或之后48小时的食物剥夺。激素处理后两天，通过心脏穿刺获得血液，上午（1100 h）一次，下午（1600-1800 h）两次。选择这些时间是为了最好地表征LH峰的幅度。食物剥夺增强了响应于雌二醇的LH峰的幅度时，这种治疗之前，但不是当它之后，管理雌二醇。然而，在发生这种食物剥夺效应的雌二醇剂量上存在变异性。在实验2中，对两组性腺完整的雌性仓鼠的运动（转轮）活动进行了定量;一组在发情周期的早期（第1 + 2天;低雌二醇）部分进行了测试，另一组在发情周期的晚期（第3 + 4天;高雌二醇）部分进行了测试。在这两组中，首先在自由进食条件下进行测试，然后在48小时的食物剥夺期间再次进行测试。平均而言，第3 + 4天组比第1 + 2天组更活跃，反映了它们内源性雌二醇的不同水平。食物剥夺显着增加自发活动，独立的发情周期期间，它被强加的阶段。这些结果进行了讨论的影响，改变雌激素受体的表达在内侧视前区可能发挥产生的影响，我们观察到短期的食物剥夺。
Two experiments investigated short-term food deprivation effects on neuroendocrine processes influenced by estrogen. These studies were prompted by prior work indicating that food deprivation increased the number of immunocytochemically identified cells containing estradiol receptors in the medial preoptic area of ovariectomized female hamsters. Presumably, this is one way that changes in metabolic fuel availability might alter the responsiveness of one or more systems to estradiol. The purpose of this study was to investigate two effects of estradiol that might be affected by food deprivation; these were 1) the positive feedback effects of estradiol on the luteinizing hormone (LH) surge, and 2) the facilitating effects of estradiol on locomotor activity. In Experiment 1, ovariectomized hamsters were administered estradiol, before or after 48 h of food deprivation. Two days after hormone treatment, blood was obtained by cardiac puncture, once in the morning (1100 h) and twice during the afternoon (1600–1800 h). These times were chosen to best characterize the magnitude of the LH surge. Food deprivation enhanced the amplitude of the LH surge in response to estradiol when this treatment preceded, but not when it followed, the administration of estradiol. However, there was variability in the dose of estradiol at which this effect of food deprivation occurred. In Experiment 2, the locomotor (running wheel) activity of two groups of gonadally intact female hamsters was quantified; one group was tested during the early (days l + 2; low estradiol) part of the estrous cycle, and the other group was tested during the late (days 3 + 4; high estradiol) part of the estrous cycle. In both groups, testing was performed first under ad lib feeding conditions and again during 48 h of food deprivation. On average, the days 3 + 4 group was more active than the days 1 + 2 group, reflecting their differing levels of endogenous estradiol. Food deprivation significantly increased locomotor activity, independently of the stage of the estrous cycle during which it was imposed. These results are discussed in terms of the influence that altered estradiol receptor expression in the medial preoptic area might play in generating the effects we observed following short-term food deprivation.