Helios but not CD226, TIGIT and Foxp3 is a Potential Marker for CD4+ Treg Cells in Patients with Rheumatoid Arthritis
Helios but not CD226, TIGIT and Foxp3 is a Potential Marker for CD4+ Treg Cells in Patients with Rheumatoid Arthritis
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DOI：
10.33594/000000080
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发表时间：
2019-04
期刊：
影响因子：
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通讯作者：
meng yang;Yan Liu;B. Mo;Youqiu Xue;Congxiu Ye;Yutong Jiang;X. Bi;Mengdan Liu;Yunting Wu
中科院分区：
文献类型：
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作者：
meng yang;Yan Liu;B. Mo;Youqiu Xue;Congxiu Ye;Yutong Jiang;X. Bi;Mengdan Liu;Yunting Wu
背景/目的：类风湿关节炎(RA)是一种进行性、慢性、甚至致残性的全身性自身免疫性疾病。致病免疫细胞和免疫抑制细胞之间的失衡与类风湿关节炎和其他自身免疫性疾病的发生发展有关。由于Foxp3在炎症存在时也表达在活化的CD4+细胞上，因此在RA患者中识别Treg细胞仍然是一个挑战。方法：采用流式细胞术进行综合分析。HELIOS、CD226、T细胞免疫受体在Ig和ITIM结构域的表达结果：我们系统地检测了可能与Treg识别相关的3个潜在标志物：Helios、CD226和TIGIT，发现在RA患者中，CD4+Foxp3+细胞上Helios的表达降低，并且与RA患者的疾病活动程度呈负相关，而CD226和TIGIT在RA患者的CD4+Foxp3+细胞中的表达水平均升高，与RA患者的疾病活动无关。结论：CD4+CD25hiCD127 low/-Foxp3+Helios+可能代表了RA患者的Treg细胞群。
Background/Aims: Rheumatoid arthritis (RA) is a progressive, chronic, even disabling systemic autoimmune disease. Imbalance between pathogenic immune cells and immunosuppressive cells is associated with the pathogenesis and development of RA and other autoimmune diseases. As Foxp3 is also expressed on activated CD4+ cells in the presence of inflammation, the identification of Treg cells in patients with RA remains a challenge. Methods: Comprehensive analyses were carried out by Flow cytometry. Expression of Helios, CD226, T cell immunoreceptor with Ig and ITIM domains clinical samples and healthy controls. Results: We have systemically examined three potential markers, Helios, CD226 and TIGIT, that are possibly related to Treg identification, and found that Helios expression on CD4+Foxp3+ cells was decreased and negatively correlated with the disease activity of RA patients, while CD226 and TIGIT both showed elevated expression levels in CD4+Foxp3+ cells in RA patients and they were not associated with disease activity of RA patients. Conclusion: Taken together, our findings indicate that CD4+CD25hiCD127low/-Foxp3+Helios+ may represent the real Treg cell population in patients with RA.