Near-infrared spectrophotometric monitoring of stroke-related changes in the protein and lipid composition of whole gerbil brains.
Near-infrared spectrophotometric monitoring of stroke-related changes in the protein and lipid composition of whole gerbil brains.
复制标题
近红外分光光度法监测整个沙鼠大脑中与中风相关的蛋白质和脂质组成的变化。
DOI：
10.1021/ac00058a003
复制
发表时间：
1993
影响因子：
7.4
通讯作者：
Lodder,RA
中科院分区：
文献类型：
--
作者：
Carney,JM;Landrum,W;Mayes,L;Zou,Y;Lodder,RA
中风是美国的一个严重问题，每年影响50多万人。由于无法持续跟踪体内缺血和再灌注过程中发生的物理和化学事件，对中风病因的研究和减少脑缺血和缺血后损伤的药物治疗试验受到挫折。本文采用近红外光谱法观察了全脑样本和完整受试者的脂质和蛋白质在中风引起的变化。通过硬件和软件技术的结合，整个大脑的检查成为可能，旨在使样品呈现在光谱仪上的重现性更高。脑组织的近红外分光光度法可以区分成人（3-4个月大）和老年（18-20个月大）的大脑，以及暴露于5- 10分钟缺血的大脑。近红外分析方法在衰老和中风研究中有许多应用，包括通过经颅获得的脑光谱无创测定年龄，脂质和蛋白质的同时多组分分析，以及水肿的量化。
Strokes are a critical problem in the US that affect more than 500 000 people annually. Re-search into the causes of strokeand testing of drug therapies to reduce ischemic and postischemic damage to the brain is frustrated by an inability to continuously follow the physical and chemical events that occur during ischemia and reperfusion in vivo. Near-IR spectrometry is used in this paper to observe stroke-induced changes inthe lipids and proteins of whole brain samples and in intact subjects. The examination of whole brains is made possible by a combination of hardware and soft-ware techniques designed to make the sample presentation to the spectrometer more reproducible. Near-IR spectrophotometry of brain tissue discriminates between adult (3-4 months of age) and aged (18-20 months of age) brains as well as between brains exposed to 5-and 10-min ischemia. The near-IR analytical method has many appli-cations in aging and stroke research, including the noninvasive determination of age from brain spectra obtained transcranially, simultaneous multicomponent analysis of lipids and proteins, and quantification of edema.