Activity, abundance and community composition of nitrite-dependent methanotrophs in response to fertilization in paddy soils
Activity, abundance and community composition of nitrite-dependent methanotrophs in response to fertilization in paddy soils
复制标题
DOI：
10.1016/j.apsoil.2021.103987
复制
发表时间：
2021-03-18
影响因子：
4.8
通讯作者：
Wu, Hong-sheng
中科院分区：
文献类型：
--
作者：
Shen, Li-dong;Liu, Jia-qi;Wu, Hong-sheng
氧化拟候选菌（Candidatus methylirabilis oxyfera， M. oxyfera）样细菌介导的亚硝酸盐依赖性甲烷厌氧氧化（AOM）具有减少水稻生态系统甲烷排放的巨大潜力。施肥是提高作物产量的必要措施，可极大地影响亚硝酸盐依赖性AOM过程。研究了不施肥对照（CK）、化学施肥（CF）和有机施肥（of） 3种施肥处理下水稻土不同层（0-10、20-30和40-50 cm）上的M. oxyfera样细菌的丰度、群落组成和活性。CF处理（6.1 × 106 ~ 2.2 × 107拷贝g-1干土）和of处理（8.9 × 106 ~ 1.8 × 107拷贝g-1干土）土壤中M. oxyfera样细菌16S rRNA基因丰度高于CK处理（5.0 × 106 ~ 1.5 × 107拷贝g-1干土）。在不同施肥处理之间，氧化铁杆菌样细菌群落有明显的分离，在CF和of土壤中，氧化铁杆菌样细菌的多样性较高。与CK （1.6 ~ 4.4 nmol CO2 g- 1（干土）d-1）处理相比，CF （3.6 ~ 11.5 nmol CO2 g- 1（干土）d-1）和OF （3.4 ~ 7.2 nmol CO2 g- 1（干土）d-1）处理的亚硝酸盐依赖性AOM活性增加，特别是在20 ~ 30 cm土层。总的来说，我们的研究结果表明，M. oxyfera样细菌的群落和活性对化学和有机施肥都有积极的响应，这扩大了我们对亚硝酸盐依赖的AOM在调节水稻生态系统甲烷排放中的作用的认识。
Nitrite-dependent anaerobic oxidation of methane (AOM), which is mediated by Candidatus Methylomirabilis oxyfera (M. oxyfera)-like bacteria, has the great potential to reduce methane emissions from paddy ecosystems. Fertilization, a necessary practice for increasing crop yields, could greatly affect nitrite-dependent AOM process. Here, we investigated the abundance, community composition and activity of M. oxyfera-like bacteria at different layers (0-10, 20-30 and 40-50 cm) of paddy soils under three fertilization treatments, including unfertilized control (CK), chemical fertilization (CF) and organic fertilization (OF). The 16S rRNA gene abundance of M. oxyfera-like bacteria in soils under CF (6.1 x 106-2.2 x 107 copies g-1 dry soil) and OF (8.9 x 106-1.8 x 107 copies g-1 dry soil) treatments was higher than that under CK treatment (5.0 x 106-1.5 x 107 copies g-1 dry soil). An obvious separation of M. oxyfera-like bacterial communities was observed between different fertilization treatments, and a higher diversity of these bacteria was detected in CF and OF soils. The nitrite-dependent AOM activity was increased in soils under CF (3.6-11.5 nmol CO2 g- 1 (dry soil) d-1) and OF (3.4-7.2 nmol CO2 g- 1 (dry soil) d-1) treatments as compared to CK (1.6-4.4 nmol CO2 g- 1 (dry soil) d-1) treatment, particularly at the layer of 20-30 cm. Overall, our results demonstrated that the communities and activity of M. oxyfera-like bacteria responded positively to both chemical and organic fertilization, which expanded our knowledge of the role of nitrite-dependent AOM in regulating methane emissions from paddy ecosystems.