HLA-DR TYPING - A COMPARISON BETWEEN NYLON WOOL ADHERENCE AND T-CELL ROSETTING IN THE ISOLATION OF B-CELLS
HLA-DR TYPING - A COMPARISON BETWEEN NYLON WOOL ADHERENCE AND T-CELL ROSETTING IN THE ISOLATION OF B-CELLS
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DOI：
10.1016/0022-1759(81)90134-4
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发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
MITTAL, KK
中科院分区：
文献类型：
--
作者：
WONG, DM;MITTAL, KK
比较了两种不同的[人] B细胞分离方法，尼龙棉粘附法与SRBC [绵羊红细胞]玫瑰花结法在B细胞纯化的技术简便性和HLA-DR表型的重现性方面的差异。从30个随机选择的供体中通过任一方法分离的B细胞在DR表型测定中给出了相同的结果。由于其简单和可靠性，尼龙棉粘附可能优于SRBC玫瑰花结法用于B细胞的常规表型分型。
A comparison was made between 2 different methods of [human] B cell isolation, nylon wool adherence vs. SRBC [sheep red blood cell] rosetting in terms of the technical ease of B cell purification and the reproducibility of HLA-DR phenotyping. B cells isolated by either method from 30 randomly selected donors gave equivalent results in the determination of DR phenotypes. Due to its simplicity and reliability, nylon wool adherence may be preferred over the SRBC rosette method for the routine phenotyping of B cells.