The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera: Trichogrammatidae)
The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera: Trichogrammatidae)
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DOI：
10.1007/s00427-017-0597-0
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发表时间：
2017-11
影响因子：
2.4
通讯作者：
Somayeh Rahimi-Kaldeh;A. Ashouri;A. Bandani;K. Tomioka
中科院分区：
文献类型：
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作者：
Somayeh Rahimi-Kaldeh;A. Ashouri;A. Bandani;K. Tomioka
在温和地区，夏末的短日照长度被用来诱导各种昆虫的滞育。许多研究表明，光周期对赤眼蜂滞育诱导具有母体效应，但目前还没有研究表明光周期与生物钟基因之间的关系。在这里，我们研究了光周期的作用滞育，生殖力，时钟基因（cry，cyc，cry2，per，和timeout）在无性和有性Trichogramma brassicae作为模式昆虫，以找到任何差异之间的两个菌株。无性系被内共生菌Wolbachia感染。短日（有性育8 h，无性育12 h）滞育率显著高于有性育。brassicae），但在所有光周期中，有性品系的滞育率均显著高于无性品系。方差分析结果表明，有性品系的钟基因表达在不同光周期之间没有显著变化，而无性品系的clk、cyc和timeout在不同光周期之间有显著变化。甘蓝型油菜显著低于有性品系。无性系的繁殖力显著高于无性系。这些结果表明，Wolbachiaininfection干扰了钟基因的表达，从而降低了滞育率，但提高了无性繁殖力。芸苔属。
The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction ofTrichogrammawasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk,cyc,cry2,per, andtimeout) in asexual and sexualTrichogramma brassicaeas a model insect to find any differences between two strains. Asexual strain was infected byWolbachia, an endosymbiont bacterium. The diapause percentage was significantly higher under short days (8 h in sexual and 12 h in the asexualT. brassicae), although the diapause percentage of the sexual strain was significantly higher than the asexual one in all the photoperiods. The ANOVA revealed no significant changes between different photoperiods in the clock gene expression in the sexual strain but significant photoperiodic changes inclk,cyc, andtimeoutin the asexual strain.Our results showed that the mRNA levels of clock genes of asexualT. brassicaewere significantly lower than those of sexual strain. The fecundity was significantly higher in the asexual strain. These results suggest thatWolbachiainfection makes disturbance on the clock gene expression which consequently reduces the percentage of diapause but increases the fecundity in asexualT. brassicae.