Selective translation during stress in Escherichia coli.
Selective translation during stress in Escherichia coli.
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DOI：
10.1016/j.tibs.2012.07.007
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发表时间：
2012-11
影响因子：
13.8
通讯作者：
Engelberg-Kulka H
中科院分区：
文献类型：
--
作者：
Moll I;Engelberg-Kulka H
众所周知，细菌应激反应是一种应对环境变化的策略，在转录水平上发挥作用。在这里，我们讨论了转录后水平的应激适应的新范例，基于最近发现的大肠杆菌中应激诱导的翻译机制修饰形式，该修饰形式由 MazF（毒素-抗毒素（TA）模块 mazEF 的毒素成分）产生。在压力下，诱导的内切核糖核酸酶 MazF 去除核糖体 16S rRNA 的 3' 端 43 个核苷酸，同时去除特定转录物的 5'-非翻译区 (UTR)。由于 MazF 对核糖体和 mRNA 的互补作用，这种优雅的机制能够实现选择性翻译，并且也代表了基于 rRNA 改变的功能性核糖体异质性的第一个例子。
The bacterial stress response, a strategy to cope with environmental changes, is generally known to operate on the transcriptional level. Here, we discuss a novel paradigm for stress adaptation at the post-transcriptional level, based on the recent discovery of a stress-induced modified form of the translation machinery in Escherichia coli that is generated by MazF, the toxin component of the toxin–antitoxin (TA) module mazEF. Under stress, the induced endoribonuclease MazF removes the 3′-terminal 43 nucleotides of the 16S rRNA of ribosomes and, concomitantly, the 5′-untranslated regions (UTRs) of specific transcripts. This elegant mechanism enables selective translation due to the complementary effect of MazF on ribosomes and mRNAs, and also represents the first example of functional ribosome heterogeneity based on rRNA alteration.