Molecular interactions of a model bile salt and porcine bile with (1,3:1,4)-β-glucans and arabinoxylans probed by 13C NMR and SAXS
Molecular interactions of a model bile salt and porcine bile with (1,3:1,4)-β-glucans and arabinoxylans probed by 13C NMR and SAXS
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DOI：
10.1016/j.foodchem.2015.10.104
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发表时间：
2016-04-15
期刊：
影响因子：
8.8
通讯作者：
Gidley, Michael J.
中科院分区：
文献类型：
--
作者：
Gunness, Purnima;Flanagan, Bernadine M.;Gidley, Michael J.
通过13 C NMR和小角X射线散射（SAXS）研究了可溶性膳食纤维（SDF）（如（1，3：1，4）-β-D-葡聚糖（β G）和阿拉伯木聚糖（AX））与胆汁盐（BS）或稀释的猪胆汁之间的相互作用。BS NMR共振的小化学位移差异与BS胶束的有效局部浓度或稀释（主要通过β G）一致，表明动态相互作用;而观察到的线宽/强度降低主要是由小麦AX和β G的最高分子尺寸和浓度引起的。SAXS显示在BS胶束的存在下β G而不是AX的变化的证据，在> 13 nm的长度尺度下与分子水平的相互作用一致。因此，SDF和BS之间的分子间相互作用取决于SDF来源及其分子量，并且可以单独或组合发生。(C)2015爱思唯尔有限公司版权所有。
Two main classes of interaction between soluble dietary fibres (SDFs), such as (1,3: 1,4)-beta-D-glucan (beta G) and arabinoxylan (AX) and bile salt (BS) or diluted porcine bile, were identified by C-13 NMR and small angle X-ray scattering (SAXS). Small chemical shift differences of BS NMR resonances were consistent with effective local concentration or dilution of BS micelles mostly by beta G, suggesting dynamic interactions; whilst the reduced line widths/intensities observed were mostly caused by wheat AX and the highest molecular size and concentrations of beta G. SAXS showed evidence of changes in beta G but not AX in the presence of BS micelles, at > 13 nm length scale consistent with molecular level interactions. Thus intermolecular interactions between SDF and BS depend on both SDF source and its molecular weight and may occur alone or in combination. (C) 2015 Elsevier Ltd. All rights reserved.