CD11b+ Peyer's patch dendritic cells secrete IL-6 and induce IgA secretion from naive B cells
CD11b+ Peyer's patch dendritic cells secrete IL-6 and induce IgA secretion from naive B cells
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DOI：
10.4049/jimmunol.171.7.3684
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发表时间：
2003-10-01
影响因子：
4.4
通讯作者：
Kaminogawa, S
中科院分区：
文献类型：
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作者：
Sato, A;Hashiguchi, M;Kaminogawa, S
与脾脏等其他淋巴器官中的树突状细胞（DC）相比，派氏集合淋巴结（PP）树突状细胞已被证明在诱导CD4(+) T细胞分泌细胞因子方面具有独特的能力。在本研究中，我们探讨了PP DC在诱导B细胞产生抗体的能力方面是否与脾脏中的DC存在功能差异。与脾脏DC相比，在体外DC - T细胞 - B细胞共培养系统中，新鲜分离的PP DC诱导初始B细胞分泌更高水平的IgA。通过中和白细胞介素 - 6（IL - 6），PP DC诱导的IgA产生减少。此外，添加外源性IL - 6可进一步增强脾脏DC而非PP DC诱导的IgA分泌。最后，我们证明只有PP CD11b(+) DC亚群与PP中的其他DC亚群以及所有脾脏DC群体相比分泌更高水平的IL - 6，并且与脾脏CD11b(+) DC相比，PP CD11b(+) DC诱导初始B细胞产生更高水平的IgA。这些结果表明PP CD11b(+) DC通过分泌IL - 6在增强B细胞产生IgA方面具有独特作用。
Peyer's patch (PP) dendritic cells (DCs) have been shown to exhibit a distinct capacity to induce cytokine secretion from CD4(+) T cells compared with DCs in other lymphoid organs such as the spleen (SP). In this study, we investigated whether PP DCs are functionally different from DCs in the SP in their ability to induce Ab production from B cells. Compared with SP DCs, freshly isolated PP DCs induced higher levels of IgA secretion from naive B cells in DC-T cell-B cell coculture system in vitro. The IgA production induced by PP DCs was attenuated by neutralization of IL-6. In addition, the induction of IgA secretion by SP DCs, but not PP DCs, was further enhanced by the addition of exogenous IL-6. Finally, we demonstrated that only PP CD11b(+) DC subset secreted higher levels of IL-6 compared with other DC subsets in the PP and all SP DC populations, and that PP CD11b(+) DC induced naive B cells to produce higher levels of IgA compared with SP CD11b(+) DC. These results suggest a unique role of PP CD11b(+) DCs in enhancing IgA production from B cells via secretion of IL-6.