High diversity and strong variation in host specificity of seed parasitic acorn weevils
High diversity and strong variation in host specificity of seed parasitic acorn weevils
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种子寄生橡实象鼻虫寄主特异性高、多样性强
DOI：
10.1111/icad.12462
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发表时间：
2020-11-19
影响因子：
3.5
通讯作者：
Chen, Xiao-Yong
中科院分区：
文献类型：
--
作者：
Li, Qian-Ya;Hu, Xing-Hua;Chen, Xiao-Yong
植食性昆虫的寄主专一性在维持植物多样性中起着重要作用。然而，缺乏方法来识别植食性昆虫的幼虫，通常具有相似的形态，可能导致误解其寄主范围。橡子象甲是壳斗科（Fagaceae）物种的种子利用寄生虫，壳斗科是亚热带万年青阔叶林（EBLF）中的优势植物群。因此，其寄主特异性的变化可能会深刻地影响该生态系统中的植物多样性。在此，我们从中国东南部EBLF中的36 655种壳斗科物种的种子中收集橡实象甲幼虫，并使用一个线粒体（COI）和两个核（ArgK和EF 1-α）基因进行多操作分类单位（OTU）划界分析，以揭示橡实象甲的物种组成，并评估OTU之间的宿主特异性差异。我们分析了来自17种常见壳斗科植物（5属）的478只橡实象甲幼虫和4只橡实象甲成虫的DNA条形码序列，并检测到11个OTU。此外，不同OTU之间的宿主范围变化很大，其中双斑是最普遍的。我们的研究结果揭示了橡子象鼻虫的高度多样性，并且宿主特异性的强烈变化甚至可以存在于同一属的OTU之间，解决了在EBLF中进行森林管理之前区分不同的橡子象鼻虫物种的需要。
Host specificity of phytophagous insects plays a critical role in the maintenance of plant diversity. However, a lack of approaches to identify the larvae of phytophagous insects, which usually have similar morphology, can lead to misinterpretation of their host ranges. Acorn weevils are seed‐exploiting parasites of Fagaceae species, a dominant plant group in subtropical evergreen broad‐leaved forests (EBLFs). Therefore, variation in their host specificity may profoundly influence plant diversity in this ecosystem. Here, we collected larvae of acorn weevils from 36 655 seeds of Fagaceae species in EBLFs in southeastern China and performed multiple operational taxonomic unit (OTU) delimitation analyses using one mitochondrial (COI) and two nuclear (ArgK and EF1‐α) genes to reveal species composition of acorn weevils and assess the variation in host specificity among OTUs. We analysed the DNA barcoding sequences from 478 larvae and 4 adults of acorn weevils sampled from 17 common Fagaceae species (five genera) and detected 11 OTUs. Moreover, host range varied drastically among different OTUs, with Curculio bimaculatus being the most generalised. Our results revealed high diversity of acorn weevils and that strong variation in host specificity can even exist among OTUs in the same genus, addressing the need to distinguish different Curculio species before conducting forest management in EBLFs.