Identification of a Prunus MAX1 homolog as a unique strigol synthase
Identification of a Prunus MAX1 homolog as a unique strigol synthase
复制标题
将 Prunus MAX1 同源物鉴定为独特的独脚金醇合酶
DOI：
10.1111/nph.19052
复制
发表时间：
2023
期刊：
影响因子：
9.4
通讯作者：
Li, Yanran
中科院分区：
文献类型：
--
作者：
Wu, Sheng;Zhou, Anqi;Hiugano, Kozue;Yoda, Akiyoshi;Xie, Xiaonan;Yamane, Kenji;Miura, Kenji;Nomura, Takahito;Li, Yanran
Strigol是最早发现的也是最重要的Strigollacone(SLS)之一，但其生物合成途径尚不清楚。我们从一组产SL的微生物群体中通过快速基因筛选从功能上鉴定了李属植物中的Strigol合成酶(细胞色素P450 711a酶)，并通过底物喂养实验和突变分析证实了其独特的催化活性(催化多步氧化)。我们还重建了烟草Strigol的生物合成途径，并报道了在大肠杆菌-酵母联合体中从简单的糖木糖中合成Strigol的情况，为大规模生产Strigoles奠定了基础。这证明了通过基因功能鉴定来成功预测植物中产生的代谢物，突显了破译植物生物合成酶序列-功能相关性的重要性，以便在不进行代谢分析的情况下更准确地预测植物代谢物。这一发现揭示了细胞色素P711A(MAX1)在SL生物合成中的进化和功能多样性，它可以合成不同立体构型的SLS(Strigol或orobancol型)。这项工作再次强调了微生物生物生产平台作为从功能上识别植物新陈代谢的有效和方便的工具的重要性。
Strigol is the first identified and one of the most important strigolactones (SLs), but the biosynthetic pathway remains elusive. We functionally identified a strigol synthase (cytochrome P450 711A enzyme) in thePrunusgenus through rapid gene screening in a set of SL‐producing microbial consortia, and confirmed its unique catalytic activity (catalyzing multistep oxidation) through substrate feeding experiments and mutant analysis.We also reconstructed the biosynthetic pathway of strigol inNicotiana benthamianaand reported the total biosynthesis of strigol in theEscherichia coli‐yeast consortium, from the simple sugar xylose, which paves the way for large‐scale production of strigol.As proof of concept, strigol and orobanchol were detected inPrunus persicaroot extrudes. This demonstrated a successful prediction of metabolites produced in plants through gene function identification, highlighting the importance of deciphering the sequence–function correlation of plant biosynthetic enzymes to more accurately predicate plant metabolites without metabolic analysis.This finding revealed the evolutionary and functional diversity of CYP711A (MAX1) in SL biosynthesis, which can synthesize different stereo‐configurations of SLs (strigol‐ or orobanchol‐type). This work again emphasizes the importance of microbial bioproduction platform as an efficient and handy tool to functionally identify plant metabolism.