Noradrenergic content and turnover rate in kidney and heart shows gender and strain differences
Noradrenergic content and turnover rate in kidney and heart shows gender and strain differences
复制标题
DOI：
10.1152/japplphysiol.00557.2001
复制
发表时间：
2002-02-01
影响因子：
3.3
通讯作者：
Ely, D
中科院分区：
文献类型：
--
作者：
Caplea, A;Seachrist, D;Ely, D
本研究的目的是比较血压正常的Wistar-Kyoto大鼠（WKY）和自发性高血压大鼠（SHR，SHR/a和SHR/y）肾脏和心脏去甲肾上腺素（NE）含量和转换率的品系和性别差异。本实验室通过对SHR/a和SHR/y两种新品系大鼠Y染色体的研究，证实Y染色体对SHR高血压模型的血压有显著影响。SHR/a具有WKY Y染色体的SHR常染色体遗传背景，而SHR/y大鼠具有WKY Y染色体的SHR常染色体遗传背景。在α-甲基-DL-β-酪氨酸注射后将组织均质化并分析NE。WKY大鼠肾脏NE含量显著低于SHR、SHR/y和SHR/a。除SHR/y外，其他品系雌性大鼠肾脏和心脏NE含量均显著高于雄性大鼠。WKY和SHR/y雌性动物的肾脏NE转换率显著低于品系匹配的雄性动物，SHR和SHR/a雌性动物的肾脏NE转换率显著高于品系匹配的雄性动物。这项研究表明，通过去甲肾上腺素能神经传递的交感神经系统活动的应变和性别差异。
The objective of this study was to compare strain and gender differences in kidney and heart norepinephrine (NE) content and turnover rate in normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR, SHR/a, and SHR/y). Our laboratory has shown that the Y chromosome has a significant effect on blood pressure in the SHR model of hypertension through the use of two new rat stains, SHR/a and SHR/y, to study the Y chromosome. SHR/a have a SHR autosomal genetic background with a WKY Y chromosome, whereas the SHR/y rats have a WKY autosomal genetic background with a SHR Y chromosome. Tissues were homogenized after alpha-methyl-DL-p-tyrosine injection and analyzed for NE. The male kidney NE content was significantly lower in the WKY compared with the SHR, SHR/y, and SHR/a. Kidney and heart NE content was significantly higher in females compared with males in all strains except the SHR/y. The WKY and SHR/y females had significantly lower kidney NE turnover rates, and the SHR and SHR/a females had significantly higher kidney NE turnover rates than strain-matched males. This study suggests both a strain and gender difference in sympathetic nervous system activity through noradrenergic neurotransmission.