EXPRESSION OF THE MESSENGER-RNA OF 7 METABOTROPIC GLUTAMATE RECEPTORS (MGLUR1 TO MGLUR7) IN THE RAT RETINA - AN IN-SITU HYBRIDIZATION STUDY ON TISSUE-SECTIONS AND ISOLATED CELLS
EXPRESSION OF THE MESSENGER-RNA OF 7 METABOTROPIC GLUTAMATE RECEPTORS (MGLUR1 TO MGLUR7) IN THE RAT RETINA - AN IN-SITU HYBRIDIZATION STUDY ON TISSUE-SECTIONS AND ISOLATED CELLS
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DOI：
10.1111/j.1460-9568.1995.tb01142.x
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发表时间：
1995-07-01
影响因子：
3.4
通讯作者：
WASSLE, H
中科院分区：
文献类型：
--
作者：
HARTVEIT, E;BRANDSTATTER, JH;WASSLE, H
我们用[α（35）S] dATP标记的寡核苷酸探针，通过组织切片和分离细胞的原位杂交，研究了7种代谢型谷氨酸受体（mGluR 1 -7）在成年大鼠视网膜中的mRNA表达。杂交结果显示6种代谢型受体mGluR 1、2和4-7在视网膜中表达，未检测到白色mGluR 3。每种表达的受体mRNA显示出不同的表达模式。在外核层，对应于感光体，没有标记检测。在内核层的外部，假定的水平细胞标记为mGluR 5。更近端在这一层，对应于双极细胞胞体的位置，有强烈的标记mGluR 6。少数双极细胞也标记了mGluR 5和mGluR 7。与分离的细胞的原位杂交显示，mGluR 6由杆双极细胞表达。亚群的无长突细胞，细胞体沿着之间的内核层和内丛状层的边界，阳性mGluR 1，2，4和7，表明这些受体之间的无长突细胞相当大的异质性。7种代谢型受体mRNA在分离的Muller神经胶质细胞中均不表达。在神经节细胞层中，几乎每个神经节细胞和移位的无长突细胞都被标记为mGluR 1和mGluR 4。该层中的一些细胞（约占总数的20%），最有可能是神经节细胞和移位的无长突细胞，也被标记为mGluR 2和mGluR 7。这些研究结果表明，代谢型谷氨酸受体是相当广泛的神经元在视网膜中比以前的生理和药理学研究表明。
We have studied the expression of mRNAs for seven metabotropic glutamate receptors (mGluR1-7) in the retina of the adult rat by in situ hybridization with tissue sections and isolated cells using [alpha(35)S]dATP-labelled oligonucleotide probes. Hybridization revealed the expression of six of the metabotropic receptor mRNAs, mGluR1, 2 and 4-7, in the retina, white mGluR3 was not detected. Each of the expressed receptor mRNAs showed a distinct pattern of expression. In the outer nuclear layer, corresponding to photoreceptor somata, no labelling was detected. In the outer part of the inner nuclear layer, putative horizontal cells were labelled for mGluR5. More proximal in this layer, corresponding to the position of bipolar cell somata, there was strong labelling for mGluR6. A small number of bipolar cells were also labelled for mGluR5 and mGluR7. In situ hybridization with isolated cells showed that mGluR6 was expressed by rod bipolar cells. Subsets of amacrine cells, with cell bodies along the border between the inner nuclear layer and the inner plexiform layer, were positive for mGluR1, 2, 4 and 7, suggesting considerable heterogeneity of these receptors among amacrine cells. None of the seven metabotropic receptor mRNAs was expressed in isolated Muller glial cells. In the ganglion cell layer, virtually every ganglion cell and displaced amacrine cell was labelled for mGluR1 and mGluR4. Some cells in this layer (-20% of the total), most likely both ganglion cells and displaced amacrine cells, were also labelled for mGluR2 and mGluR7. These findings suggest that metabotropic glutamate receptors are considerably more widespread among neurons in the retina than indicated by previous physiological and pharmacological investigations.