Identification of glycoproteins specific to biotrophic intracellular hyphae formed in the Colletotrichum lindemuthianum-bean interaction.
Identification of glycoproteins specific to biotrophic intracellular hyphae formed in the Colletotrichum lindemuthianum-bean interaction.
复制标题
鉴定在炭疽菌-豆相互作用中形成的生物营养型细胞内菌丝特异的糖蛋白。
DOI：
10.1111/j.1469-8137.1994.tb04275.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
J. R. Green
中科院分区：
文献类型：
--
作者：
N. Pain;R. O’Connell;K. Mendgen;J. R. Green
针对菌丝(IH，即感染小泡和初级菌丝)的细胞内特异性单抗(MAb)。炭疽菌(Colletotrichum lindemuthianum(Sce，&Magn.)Briosi&Cav.用联合免疫的方法从四季豆病叶中分离到。免疫荧光免疫金标和免疫印迹分析表明，UB25对分生孢子、分生孢子芽管、附着体或体外生长的附着体芽管没有亲和力，对任何植物成分都没有亲和力。用高压冷冻、冷冻置换、低温包埋等方法制备的感染组织免疫金标记法表明，UB25抗原存在于IH周围的界面基质和真菌壁中。该抗原仅局限于侵染囊泡和与寄主原生质体接触的初生菌丝中，在生长在胞间的初生菌丝中检测不到该抗原。UB25识别存在于一组N-连接的糖蛋白中的蛋白质表位。这些糖蛋白在细胞内发育的早期阶段表达，表明可能在生物营养或识别中发挥作用。
Monoclonal antibodies (MAbs) specific for intracellular for hyphae (IH, i.e. infection vesicles and primary hyphae). Appressoria/germ tubes and conidia of Colletotrichum lindemuthianum (Sace, & Magn.) Briosi & Cav. isolated from infected leaves of Phaseolus vulgoria L. were obtained using a co-immunization procedure. One of the MAhs; UB25, bound specifically to IH in immunofluorescence immunogold and Western blot assays: it showed no affinity for conidia, conidial germ tubes, appressoria or appressorial germ tubes growing in vitro, of for any plant components. Immunogold labeling of infected tissue prepared by high pressure freezing, freeze-substitution and low temperature embedding showed that the UB25 antigen was present in the interfacial matri surrounding IH and in the fungal wall. The antigen was confined to infection vesicles and primary hyphae in contact with host protoplast and could not be detected in primary hyphae growing in intercellular spaces. UB25 recognizes a protein epitope present in a set of N-linked glycoproteins. These glycoproteins are expressed at an early stage of intracellular development, suggesting a possible role in biotrophy or recognition.