Determination of the DNA sugar pucker using 13C NMR spectroscopy.
Determination of the DNA sugar pucker using 13C NMR spectroscopy.
复制标题
使用 13C NMR 光谱法测定 DNA 糖褶皱。
DOI：
10.1021/bi00450a018
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Harbison,GS
中科院分区：
文献类型：
--
作者：
Santos,RA;Tang,P;Harbison,GS
纽约州立大学化学系，石溪，纽约，11794收到6月6日，；修订稿件收到7月18日，摘要：固体13C核磁共振波谱的一系列晶体核苷酸和核苷酸可以直接测量脱氧核糖环构象对碳化学位移的影响。研究发现，3‘-endo构象与3’-exo构象和2‘-endo构象相比，具有显著的3’和5‘化学位移(5-10ppm)。后两种构象的区别可能是C-2‘和C-4’位置的碳化学位移较小，但仍有显著差异。对纤维状小牛胸腺DNA的三种修饰的高分辨固体核磁共振谱表明，这些趋势在高分子量DNA中保持不变，并证实A-DNA中的主要环折叠子是3‘-endo，而B-DNA和C-DNA主要是2，-endo。结果表明，~(13)C核磁共振谱是一种直观、实用的DNA环状折叠探针。
Department of Chemistry, State University of New York, Stony Brook, New York 11794 Received June 6, 1989; Revised Manuscript Received July 18, 1989 abstract: Solid-state 13C NMR spectroscopy of a series of crystalline nucleosides and nucleotides allows direct measurement of the effect of the deoxyribose ring conformation on the carbon chemical shift. It is found that 3'-endo conformers have 3'and 5'chemical shifts significantly (5-10 ppm) upfield of comparable 3'-exo and 2'-endo conformers. The latter two conformers may be distinguished by smaller but still significant differences in the carbon chemical shifts at the C-2'and C-4'positions. High-resolution solid-state NMR of three modifications of fibrous calf thymus DNA shows that these trends are maintained in high-molecular-weight DNA and confirmsthat the major ring pucker in A-DNA is 3'-endo, while both B-DNA and C-DNA are largely 2,-endo. The data show that 13C NMR spectroscopy is a straightforward and useful probe of DNA ring pucker in both solution and the solid state.