ORAL IMMUNIZATION WITH A RECOMBINANT BACTERIAL-ANTIGEN PRODUCED IN TRANSGENIC PLANTS
ORAL IMMUNIZATION WITH A RECOMBINANT BACTERIAL-ANTIGEN PRODUCED IN TRANSGENIC PLANTS
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DOI：
10.1126/science.7732379
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发表时间：
1995-05-05
期刊：
影响因子：
56.9
通讯作者：
ARNTZEN, CJ
中科院分区：
文献类型：
--
作者：
HAQ, TA;MASON, HS;ARNTZEN, CJ
大肠杆菌不耐热肠毒素（LT-B）结合亚单位是一种高活性的口服免疫原。使用编码LT-B或具有微粒体保留序列的LT-B融合蛋白的基因制备转基因烟草和马铃薯植物。植物表达了外源肽，两者都形成了与天然配体结合的寡聚体。通过灌胃免疫的小鼠产生血清和肠粘膜抗LT-B免疫球蛋白，其在细胞保护测定中中和肠毒素。给小鼠喂食新鲜的转基因马铃薯块茎也引起口服免疫。
The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.