Isolation of Photobacterium sp LuB-1 and its application in rapid assays for chemical toxicants in water
Isolation of Photobacterium sp LuB-1 and its application in rapid assays for chemical toxicants in water
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发光杆菌LuB-1的分离及其在水中化学毒物快速检测中的应用
DOI：
10.1111/j.1472-765x.2010.02896.x
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发表时间：
2010-09-01
影响因子：
2.4
通讯作者：
Zhai, Q.
中科院分区：
文献类型：
--
作者：
Hong, Y.;Chen, Z.;Zhai, Q.
目的：从中国体内分离到一株发光细菌，命名为Lub-1，用于筛选在盐浓度范围内，特别是在低盐条件下具有较强发光能力的海洋细菌。经生化分析和基于16S rRNA基因的系统发育分析，鉴定为发光菌属。该材料在0、中心点2~5%(w/v)的氯化钠浓度范围内均能发出强而稳定的发光。在本研究所测试的大多数毒物中，Lub-1菌株在低盐(0中心点9%NaC l)和高盐(2中心点0%NaC l)条件下的响应均好于Microtox(TM)费氏弧菌。结论：Lub-1菌株在较宽的盐浓度范围内具有明显的生物发光优势，在低盐和高盐毒性测试系统中对重金属污染物和某些有机毒物都有较好的响应。Lub-1菌株在不同盐度的水中毒物的快速检测中应具有独特的优势。
Aims:To isolate marine bacteria with strong bioluminescence in a wide range of NaCl concentration, especially at low salt conditions.Methods and Results:A luminous bacterium named LuB-1 was isolated from China. It was identified by biochemical analysis and phylogenetic analysis based on the 16S rRNA gene and designated as Photobacterium sp. The isolate is capable of emitting strong and stable luminescence in a wide range of NaCl concentration from 0 center dot 2 to 5% (w/v). For most toxic agents tested in this study, the response of LuB-1 was better than that of Microtox (TM) Vibrio fischeri under both low salt (0 center dot 9% NaCl) and high salt (2 center dot 0% NaCl) conditions.Conclusion:The strain LuB-1 had an obvious predominance of bioluminescence in a wide range of NaCl concentration and better response for heavy metal pollutants and some organic toxicants in both low and high salt toxicity test systems.Significance and Impact of the Study:Because of its good sensitivity in a wide range of salt concentration, the strain LuB-1 should have its unique advantage in rapid assay for toxicants in water with different salt concentrations.