spa Typing method for discriminating among Staphylococcus aureus isolates:: Implications for use of a single marker to detect genetic micro- and macrovariation
spa Typing method for discriminating among Staphylococcus aureus isolates:: Implications for use of a single marker to detect genetic micro- and macrovariation
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DOI：
10.1128/jcm.42.2.792-799.2004
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发表时间：
2004-02-01
影响因子：
9.4
通讯作者：
Kreiswirth, BN
中科院分区：
文献类型：
--
作者：
Koreen, L;Ramaswamy, SV;Kreiswirth, BN
微生物病原体的菌株分型有两个主要目的：（i）指示遗传微变异。用于爆发调查和（ii）索引遗传宏观变异用于系统发育和基于人群的分析。到目前为止，还没有明确的迹象表明，一个遗传标记可以有效地用于这两个目的。以前，我们已经表明，蛋白A基因可变重复区的DNA序列分析（SPA分型）提供了一种快速，准确的方法来区分金黄色葡萄球菌爆发分离株与那些被认为流行病学无关。在这里，使用低水平重组物种内的遗传宏观变异将准确地由单位点标记表征的假设，我们测试了spa分型是否可以一致地索引由全基因组DNA微阵列检测到的广泛的遗传变异。这代表了S内部多样性的广度。金黄色。利用SPA和CoA分型、脉冲场凝胶电泳（PFGE）、微阵列和多位点酶电泳（MLEE）数据进行分子流行病学和进化分析，我们确定了S。金黄色葡萄球菌可能具有主要的克隆群体结构，并且SPA分型可以单独指示遗传变异，与微阵列具有88%的直接一致性，并且可以正确地将分离株分配到系统发育谱系。SPA分型在区分能力和与微阵列在不同系统发育深度的一致程度方面优于MLEE、PFGE和CoA分型。这项研究表明，蛋白A重复区的遗传分析全面表征了S的主要克隆群体结构的微观和宏观变异。金黄色。
Strain typing of microbial pathogens has two major aims: (i) to index genetic microvariation for. use in outbreak investigations and (ii) to index genetic macrovariation for use in phylogenetic and population-based analyses. Until now, there has been no clear indication that one genetic marker can efficiently be used for both purposes. Previously, we had shown that DNA sequence analysis of the protein A gene variable repeat region (spa typing) provides a rapid and accurate method to discriminate Staphylococcus aureus outbreak isolates from those deemed epidemiologically unrelated. Here, using the hypothesis that the genetic macrovariation within a low-level recombinogenic species would accurately be characterized by a single-locus marker, we tested whether spa typing could congruently index the extensive genetic variation detected by a whole-genome DNA microarray in a collection of 36 isolates, which was recovered from 10 countries on four continents over a period of four decades, that is representative of the breadth of diversity within S. aureus. Using spa and coa typing, pulsed-field gel electrophoresis (PFGE), and microarray and multilocus enzyme electrophoresis (MLEE) data in molecular epidemiologic and evolutionary analyses, we determined that S. aureus likely has a primarily clonal population structure and that spa typing can singly index genetic variation with 88% direct concordance with the microarray and can correctly assign isolates to phylogenetic lineages. spa typing performed better than MLEE, PFGE, and coa typing in discriminatory power and in the degree of agreement with the microarray at various phylogenetic depths. This study showed that genetic analysis of the repeat region of protein A comprehensively characterizes both micro- and macrovariation in the primarily clonal population structure of S. aureus.