Temporal single-cell transcriptomes of zebrafish spinal cord pMN progenitors reveal distinct neuronal and glial progenitor populations.
Temporal single-cell transcriptomes of zebrafish spinal cord pMN progenitors reveal distinct neuronal and glial progenitor populations.
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DOI：
10.1016/j.ydbio.2021.07.010
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发表时间：
2021-11
影响因子：
2.7
通讯作者：
Appel B
中科院分区：
文献类型：
--
作者：
Scott K;O'Rourke R;Winkler CC;Kearns CA;Appel B
表达碱性螺旋环螺旋转录因子Olig 2的脊髓前体细胞依次产生运动神经元和少突胶质细胞前体细胞（OPCs）。以下规格，一些OPCs分化为髓鞘少突胶质细胞，而其他人坚持作为OPCs。虽然相当多的工作已经描述了运动神经元，OPCs和少突胶质细胞的分子特征，但对产生它们的祖细胞知之甚少。为了确定运动神经元和OPC的发育起源和转录谱，我们对胚胎斑马鱼躯干组织中分离的pMN细胞进行了单细胞RNA测序，包括运动神经发生，OPC规范和少突胶质细胞分化的启动。下游分析揭示了两个不同的pMN祖细胞群：一个似乎产生神经元，一个似乎产生OPCs。这后一个群体，称为前-OPC，是由GS同源框2（gsx 2），一个基因，编码同源框转录因子的表达为标志。使用荧光原位杂交，我们确定了gsx 2表达前OPC在脊髓中的典型OPC标记基因的表达。因此，我们的数据揭示了异质性基因表达谱之间的pMN祖细胞，支持以前的命运作图证据。
Ventral spinal cord progenitor cells, which express the basic helix loop helix transcription factor Olig2, sequentially produce motor neurons and oligodendrocyte precursor cells (OPCs). Following specification some OPCs differentiate as myelinating oligodendrocytes while others persist as OPCs. Though a considerable amount of work has described the molecular profiles that define motor neurons, OPCs, and oligodendrocytes, less is known about the progenitors that produce them. To identify the developmental origins and transcriptional profiles of motor neurons and OPCs, we performed single-cell RNA sequencing on isolated pMN cells from embryonic zebrafish trunk tissue at stages that encompassed motor neurogenesis, OPC specification, and initiation of oligodendrocyte differentiation. Downstream analyses revealed two distinct pMN progenitor populations: one that appears to produce neurons and one that appears to produce OPCs. This latter population, called Pre-OPCs, is marked by expression of GS Homeobox 2 (gsx2), a gene that encodes a homeobox transcription factor. Using fluorescent in situ hybridizations, we identified gsx2-expressing Pre-OPCs in the spinal cord prior to expression of canonical OPC marker genes. Our data therefore reveal heterogeneous gene expression profiles among pMN progenitors, supporting prior fate mapping evidence.
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影响因子：
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作者：
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