IDENTIFICATION OF GENES INVOLVED IN DNA-REPLICATION OF THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS
IDENTIFICATION OF GENES INVOLVED IN DNA-REPLICATION OF THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS
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DOI：
10.1073/pnas.91.23.11212
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发表时间：
1994-11-08
影响因子：
11.1
通讯作者：
VLAK, JM
中科院分区：
文献类型：
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作者：
KOOL, M;AHRENS, CH;VLAK, JM
通过使用瞬时复制试验，在苜蓿银纹夜蛾杆状病毒基因组中鉴定了9个参与DNA复制的基因。编码解旋酶、DNA聚合酶、IE-1、LEF-1、LEF-2和LEF-3的六个基因是DNA复制所必需的，而编码P35、IE-2和PE 38的三个基因刺激DNA复制。AcMNPV pcna基因编码的蛋白质序列同源性增殖细胞核抗原，没有刺激，观察。在IE-1的羧基末端区域鉴定了在许多单链DNA结合蛋白中发现的氨基酸模式。
By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-I, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the AcMNPV pcna gene, encoding a protein with sequence homology to proliferating-cell nuclear antigen, was observed. A pattern of amino acids found in a number of single-stranded-DNA-binding proteins was identified in the carboxyl-terminal region of IE-1.