SILENT PSEUDOCHOLINESTERASE GENE
SILENT PSEUDOCHOLINESTERASE GENE
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DOI：
10.1038/193561a0
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发表时间：
1962-01-01
期刊：
影响因子：
64.8
通讯作者：
LEHMANN, H
中科院分区：
文献类型：
--
作者：
LIDDELL, J;SILK, E;LEHMANN, H
每千名住院病人中约有0.25 -1人可被证明具有由遗传决定的血清假胆碱酯酶缺乏症。这些受试者通常被发现，因为他们对短效肌肉松弛剂琥珀胆碱表现出明显的敏感性。这种药物是琥珀酸的二胆碱酯，被假胆碱酯酶迅速水解。Kalow和他的同事已经证明，在这些罕见的个体中发现的酶在两个方面不同于通常的类型：它对各种各样的底物的效果较差，对大多数底物的抗性更强3，4。Kalow和Genest设计了一种试验系统，使用5 × 10-5 M苯甲酰胆碱作为底物，10-5 M浓度的局部麻醉剂地布卡因（'Nuperestine'）作为抑制剂”。在这些条件下，地布卡因抑制了大约80%的常见酶，而琥珀胆碱敏感个体的血清则是
ABOUT 0'25-1 per mille of hospital patients can be shown to have a genetically determined deficiency of serum pseudocholinesterase. These subjects are usually discovered because they show a marked sensitivity to the short-acting muscle relaxant, suxamethonium1• This drug, which is the dicholine ester of succinic acid, is rapidly hydrolysed by pseudocholinesterase'.Kalow and his colleagues have shown that the enzyme found in these rare individuals differs from the usual type in two respects: it is less effective against a wide range of substrates, and more resistant to the majority of inhibitors3, 4. Kalow and Genest devised a test system using 5 x 10-5 M benzoylcholine as a substrate and a 10-5 M concentration of the local anresthetic dibucaine ('Nupercaine') as an inhibitor". Under these conditions, dibucaine inhibits the usual enzyme by about 80 per cent, whereas sera from the suxamethonium-sensitive individuals are