Cold-regulated genes under control of the cold sensor Hik33 in Synechocystis
Cold-regulated genes under control of the cold sensor Hik33 in Synechocystis
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DOI：
10.1046/j.1365-2958.2001.02379.x
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发表时间：
2001-04-01
影响因子：
3.6
通讯作者：
Murata, N
中科院分区：
文献类型：
--
作者：
Suzuki, I;Kanesaki, Y;Murata, N
组氨酸激酶，Hik 33，似乎感温度下降，并调节某些冷诱导基因的表达，在蓝藻集胞藻属。PCC 6803。为了研究Hik 33在基因表达调控中的作用，我们使用DNA微阵列技术分析了Delta Hik 33突变体。在野生型细胞中，在低温下被强烈诱导的基因编码的蛋白质主要是转录和翻译机器的亚基。大多数冷抑制基因编码光合作用机制的组成部分。hik 33基因的突变抑制了部分冷调节基因的表达，根据hik 33基因突变的影响可将其分为三组。在第一组中，低温对基因表达的调节完全消失;在第二组中，这种调节的程度减少了一半;最后，在第三组中，这种调节完全不受影响。这些结果表明，第一组中的基因的表达仅由Hik 33调节，第三组中的基因的表达由尚未鉴定的冷传感器调节，第二组中的基因的末端表达由这两种冷传感器调节。
A histidine kinase, Hik33, appears to sense decreases in temperature and to regulate the expression of certain cold-inducible genes in the cyanobacterium Synechocystis sp. PCC6803. To examine the role of Hik33 in the regulation of gene expression, we analysed a Delta Hik33 mutant using the DNA microarray technique. In wild-type cells, genes that were strongly induced at low temperature encoded proteins that were predominantly subunits of the transcriptional and translational machinery. Most cold-repressible genes encoded components of the photosynthetic machinery. Mutation of the hik33 gene suppressed the expression of some of these cold-regulated genes, which could be divided into three groups according to the effect of the mutation of hik33. In the first group, regulation of gene expression by low temperature was totally abolished; in the second group, the extent of such regulation was reduced by half; end, in the third group, such regulation was totally unaffected. These results suggest that expression of the genes in the first group is regulated solely by Hik33, expression of genes in the third group is regulated by an as yet unidentified cold sensor, end expression of genes in the second group is regulated by both these cold sensors.