The keratan sulfate-enriched region of bovine cartilage proteoglycan consists of a consecutively repeated hexapeptide motif.
The keratan sulfate-enriched region of bovine cartilage proteoglycan consists of a consecutively repeated hexapeptide motif.
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牛软骨蛋白聚糖富含硫酸角质素的区域由连续重复的六肽基序组成。
DOI：
10.1016/s0021-9258(18)71603-7
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Åke Oldberg
中科院分区：
文献类型：
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作者：
P. Antonsson;D. Heinegård;Åke Oldberg
我们已经确定了编码大聚集软骨蛋白聚糖核心蛋白的富含硫酸角质素结构域的cDNA克隆的序列。推导的氨基酸序列的 C 端部分与大鼠软骨肉瘤蛋白多糖的硫酸软骨素富含区（结构域 CS1）同源，N 端部分与大鼠蛋白多糖的第二球状结构域（G2）同源（Doege, K.、Sasaki, M.、Horigan, E.、Hassell, J. R. 和 Yamada, Y. (1987) 化学。 262, 17757–17767)。我们可以识别插入这些区域之间的大鼠蛋白多糖中不存在的区域。该结构域对应于牛蛋白聚糖的富含硫酸角质素的区域。它由连续重复23次的高度保守的六肽基序组成。基因组 DNA 的转移印迹分析表明是单个基因。硫酸角质素富集区的编码区存在于人类和牛 DNA 中，而该结构域的编码区似乎不存在于大鼠基因组中。 RNA 的转移印迹分析表明，富含硫酸角质素的区域存在于胎儿和成人来源的蛋白聚糖中。此外，对从成年和胎儿牛关节软骨中分离的RNA进行的RNA保护分析表明，在硫酸角质素富集的区域内没有发生选择性剪接。这些实验表明胎牛软骨蛋白多糖含有硫酸角质素附着结构域，尽管它缺乏硫酸角质素侧链。
We have determined the sequence of a cDNA clone encoding the keratan sulfate-rich domain of the large aggregating cartilage proteoglycan core protein. The C-terminal portion of the deduced amino acid sequence is homologous to the chondroitin sulfate-rich region (domain CS1) of the rat chondrosarcoma proteoglycan, and the N-terminal portion is homologous to the second globular domain (G2) of the rat proteoglycan (Doege, K., Sasaki, M., Horigan, E., Hassell, J. R., and Yamada, Y. (1987)J. Biol. Chem. 262, 17757–17767). We could identify, inserted between these regions, a region absent in the rat proteoglycan. This domain corresponds to the keratan sulfate-enriched region of the bovine proteoglycan. It consists of a highly conserved hexapeptide motif consecutively repeated 23 times. Transfer blot analysis of genomic DNA indicated a single gene. The coding region for the keratan sulfate-enriched region was present both in human and bovine DNA, whereas the coding region for this domain appears to be absent in the rat genome. Transfer blot analysis of RNA showed that the keratan sulfate-rich region is present in proteoglycans from fetal as well as adult sources. Furthermore, RNA protection assays of RNA isolated from adult and fetal bovine articular cartilage showed that no alternative splicing occurs within this keratan sulfate-enriched region. These experiments show that the fetal bovine cartilage proteoglycan contains the keratan sulfate attachment domain, although it lacks the keratan sulfate side chains.