Comparative metabolomics approach coupled with cell- and gene-based assays for species classification and anti-inflammatory bioactivity validation of Echinacea plants
Comparative metabolomics approach coupled with cell- and gene-based assays for species classification and anti-inflammatory bioactivity validation of Echinacea plants
复制标题
DOI：
10.1016/j.jnutbio.2009.08.010
复制
发表时间：
2010-11-01
影响因子：
5.6
通讯作者：
Shyur, Lie-Fen
中科院分区：
文献类型：
--
作者：
Hou, Chia-Chung;Chen, Chun-Houh;Shyur, Lie-Fen
在过去的十年中，紫锥菊制剂是最畅销的草药补充剂或药物；然而，在商业紫锥菊产品中仍然经常错误识别或替代紫锥菊植物物种，在特定制剂中没有明确的化学成分。在本报告中，结合超临界流体萃取、气相色谱/质谱仪和数据挖掘的比较代谢组学研究表明，根据代谢产物的分布和相对含量，可以很容易地将紫锥菊、青紫锥菊和狭叶紫锥菊这三种最常用的药用紫锥菊进行分类。一项丝裂原诱导的小鼠皮肤炎症研究表明，烷胺类化合物是紫锥菊植物中存在的活性抗炎成分。然后从紫茎根提取物中分离得到混合烷酰胺类化合物和主要成分十二烷基二烯酸异丁酰胺(8/9)，用于进一步的生物活性鉴定。在巨噬细胞中，烷胺类化合物显著抑制环氧合酶2(COX-2)活性和脂多糖诱导的COX-2、诱导型一氧化氮合酶和特异性细胞因子或趋化因子[即肿瘤坏死因子α、白介素1α、IL-6、MCP-1、MIP-1β]的表达，而上调血红素加氧酶-1蛋白的表达。菊苣酸，然而，显示出很少或没有影响。高效液相色谱/电子喷雾电离/质谱联用法测定紫茎泽兰根部的烷胺类化合物和酚类化合物的结果表明，在一定的采后或非生物处理条件下，特定的植物类化合物(即烷胺类、菊苣酸和芦丁)的含量会发生变化。这项研究为使用新兴的代谢组学方法与生物活性分析相结合，用于药用/营养植物种类分类、质量控制和鉴定治疗炎症性疾病的新型植物制剂提供了新的见解。
Echinacea preparations were the top-selling herbal supplements or medicines in the past decade; however, there is still frequent misidentification or substitution of the Echinacea plant species in the commercial Echinacea products with not well chemically defined compositions in a specific preparation. In this report, a comparative metabolomics study, integrating supercritical fluid extraction, gas chromatography/mass spectrometry and data mining, demonstrates that the three most used medicinal Echinacea species, Echinacea purpurea, E. pallida, and E. angustifolia, can be easily classified by the distribution and relative content of metabolites. A mitogen-induced murine skin inflammation study suggested that alkamides were the active anti-inflammatory components present in Echinacea plants. Mixed alkamides and the major component, dodeca-2E,4E,8Z,10Z(E)-tetraenoic acid isobutylamides (8/9), were then isolated from E. purpurea root extracts for further bioactivity elucidation. In macrophages, the alkamides significantly inhibited cyclooxygenase 2 (COX-2) activity and the lipopolysaccharide-induced expression of COX-2, inducible nitric oxide synthase and specific cytokines or chemokines [i.e., TNF-alpha, interleukin (IL)-1 alpha, IL-6, MCP-1, MIP-1 beta] but elevated heme oxygenase-1 protein expression. Cichoric acid, however, exhibited little or no effect. The results of high-performance liquid chromatography/electron spray ionization/mass spectrometry metabolite profiling of alkamides and phenolic compounds in E. purpurea roots showed that specific phytocompound (i.e., alkamides, cichoric acid and rutin) contents were subject to change under certain post-harvest or abiotic treatment. This study provides new insight in using the emerging metabolomics approach coupled with bioactivity assays for medicinal/nutritional plant species classification, quality control and the identification of novel botanical agents for inflammatory disorders.