REFERENCE MATERIAL FOR NUTRIENTS IN SEAWATER - STABILITY OF NITRATE, NITRITE, AMMONIA AND PHOSPHATE IN AUTOCLAVED SAMPLES
REFERENCE MATERIAL FOR NUTRIENTS IN SEAWATER - STABILITY OF NITRATE, NITRITE, AMMONIA AND PHOSPHATE IN AUTOCLAVED SAMPLES
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DOI：
10.1016/0304-4203(95)00004-b
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发表时间：
1995-04-01
期刊：
影响因子：
3
通讯作者：
KEROUEL, R
中科院分区：
文献类型：
--
作者：
AMINOT, A;KEROUEL, R
标准物质越来越多地被用作一般质量控制和质量保证的基础。介绍并讨论了高压灭菌用于制作海水中营养物质标准物质的结果。当初步实验表明，硝酸盐、亚硝酸盐和氨氮在海水样品中高压灭菌时表现出满意的行为时，对这三种营养物质和三种浓度的磷酸盐进行了27个月的实验。因此，使用高压灭菌法稳定了ICES第五次内部比对活动的样品，并在19个月期间进行了检查。氮素营养在样品间表现出最大的均质性。其中，硝酸盐和亚硝酸盐在整个实验过程中非常稳定，总的标准偏差分别低于0.3%(范围为5-50mU·L(-1))和0.8%(范围为0.5-5mU·m-L(-1))。对于这两种营养物质，高压灭菌似乎是在海水中生产标准物质的主要方法。氨随着时间的推移表现出轻微的增加，每年高达0.05-0.07mmolL(-1)，归因于样品不能充分保护实验室大气而造成的空气污染。由于容器玻璃的淋洗，磷酸盐每年增加0.02-0.07万摩尔L(-1)。尽管有这些轻微的不利影响，高压灭菌法也可以用于氨和磷酸盐，在适当的、可控的条件下，储存期更短。磷酸盐问题，与稳定过程本身无关，可能可以通过使用更惰性的容器来解决。
Reference material is increasingly used as a basis for quality control and quality assurance in general. Results of autoclaving applied to produce reference material for nutrients in seawater are presented and discussed.When preliminary experiments had shown satisfactory behavior of nitrate, nitrite and ammonia when autoclaved together in seawater samples, a 27 month experiment was undertaken with these three nutrients and phosphate at three concentration levels. Autoclaving was therefore used to stabilize the samples for the ICES 5th intercomparison exercise and checked over a 19 month period. Nitrogenous nutrients exhibited the greatest inter-sample homogeneity. Among them, nitrate and nitrite were extremely stable throughout the whole experiment with overall standard deviations lower than 0.3% (range 5-50 mu mol l(-1)) and 0.8% (range 0.5-5 mu mol l(-1)), respectively. For these two nutrients, autoclaving appears to be a prime method for production of reference material in seawater. Ammonia exhibited a slight increase over time, up to 0.05-0.07 mu mol l(-1) per year, attributed to air contamination when samples were insufficiently protected from the laboratory atmosphere. Phosphate increased by 0.02-0.07 mu mol l(-1) per year due to leaching from the container glass. Despite these slight adverse effects, autoclaving may also be used for ammonia and phosphate with shorter periods of storage under adequate, controlled conditions. The phosphate problem, not pertaining to the stabilization process itself, can probably be solved by using a more inert container.