Regulated nuclear translocation of the Mig1 glucose repressor
Regulated nuclear translocation of the Mig1 glucose repressor
复制标题
DOI：
10.1091/mbc.8.8.1603
复制
发表时间：
1997-08-01
影响因子：
3.3
通讯作者：
Johnston, M
中科院分区：
文献类型：
--
作者：
DeVit, MJ;Waddle, JA;Johnston, M
葡萄糖抑制面包酵母（酿酒酵母）中许多基因的转录。Mig 1是一种Cys（2）-His（2）锌指蛋白，通过结合其启动子并募集一般阻遏复合物Ssn 6-Tup 1来介导几个基因的葡萄糖阻遏。我们发现Mig 1的亚细胞定位受葡萄糖的调节。Mig 1在加入葡萄糖后几分钟内被导入细胞核，并且在去除葡萄糖后同样迅速地被转运回细胞质。这种受调节的核定位需要葡萄糖阻遏信号转导途径的组分。与DNA结合和抑制结构域分离的蛋白质内部区域对于葡萄糖调节的核输入和输出是必要的和足够的。Mig 1磷酸化状态的变化与其定位的变化一致，表明磷酸化可能具有调节作用。我们的研究结果表明，葡萄糖调节的核进口和/或出口机制控制Mig 1的活动。
Glucose represses the transcription of many genes in bakers yeast (Saccharomyces cerevisiae). Mig1 is a Cys(2)-His(2) zinc finger protein that mediates glucose repression of several genes by binding to their promoters and recruiting the general repression complex Ssn6-Tup1. We have found that the subcellular localization of Mig1 is regulated by glucose. Mig1 is imported into the nucleus within minutes after the addition of glucose and is just as rapidly transported back to the cytoplasm when glucose is removed. This regulated nuclear localization requires components of the glucose repression signal transduction pathway. An internal region of the protein separate from the DNA binding and repression domains is necessary and sufficient for glucose-regulated nuclear import and export. Changes in the phosphorylation status of Mig1 are coincident with the changes in its localization, suggesting a possible regulatory role for phosphorylation. Our results suggest that a glucose-regulated nuclear import and/or export mechanism controls the activity of Mig1.