Dehydroalanine-Based Diubiquitin Activity Probes
Dehydroalanine-Based Diubiquitin Activity Probes
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DOI：
10.1021/ol403416w
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发表时间：
2014-01-17
期刊：
影响因子：
5.2
通讯作者：
Brik, Ashraf
中科院分区：
文献类型：
--
作者：
Haj-Yahya, Najat;Hemantha, Hosahalli P.;Brik, Ashraf
描述了一种合成脱氢丙氨酸的二氨基素活性探针的策略。脱氢丙氨酸的特定于位置的引入是从二醇轴承蛋白轴承残留物附近的二氨基蛋白轴承蛋白附近获得的，这些残留物在两个通过Lys48，Lys63或以尾部方式连接的泛素蛋白之间。这些探针的特征是其各种去泛素酶的活动，这些探针在研究各种环境中研究去泛素酶方面开辟了新的机会。
A strategy for the synthesis of dehydroalanine based diubiquitin activity probes is described. The site-specific introduction of dehydroalanine was achieved from diubiquitin bearing Cys residue near the scissile bond between two ubiquitins linked through Lys48, Lys63 or in a head to tail fashion. The probes were characterized for their activities with various deubiquitinases, which open new opportunities in studying deubiquitinases in various settings.