Blood-sample processing for the study of age-dependent gene expression in peripheral blood mononuclear cells.
Blood-sample processing for the study of age-dependent gene expression in peripheral blood mononuclear cells.
复制标题
用于研究外周血单核细胞中年龄依赖性基因表达的血液样本处理。
DOI：
10.1093/gerona/57.7.b285
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Wang,Eugenia
中科院分区：
文献类型：
--
作者：
Lacelle,Chantale;Riol,Hélène;Xu,Suying;Tang,Yih-Jing;Wang,Yuh-Shyun;Chuang,Yi-Li;Lin,Hui-Sheng;Chang,Ming-Cheng;Liang,Jersey;Wang,Eugenia
尽管大多数新的生物老年学研究寻求识别与人类成功衰老有关的长寿候选基因和因素，这些研究都是以人群为基础的，并且可能涉及从极其年老的个体中采集血液，但据我们所知，尚未发布统一的方案来描述一种方法，该方法允许同时生成来自单一来源的极少量外周血的不同种类的生物样本。在这里，我们描述了一种方法，允许从任何年龄（10-102岁）的献血者获得的单个10至30毫升血液样本中同时生成血浆、RNA、DNA、蛋白质、固定淋巴细胞和冷冻血液等分试样，并且我们表明所获得的DNA、RNA、蛋白质和固定淋巴细胞的质量和数量不会随年龄而显着变化。正如经常观察到的那样，老年人的血浆比例较高。
Although most new biogerontological studies seeking to identify longevity candidate genes and factors involved in successful human aging are population based, and likely to involve the collection of blood from extremely old individuals, to our knowledge no unified protocols have yet been published to describe a methodology permitting the simultaneous generation of different kinds of biological specimens derived from a single source of a very small volume of peripheral blood. Here we describe a method permitting the simultaneous generation of plasma, RNA, DNA, protein, fixed lymphocytes, and frozen blood aliquots from a single 10- to 30-ml blood sample obtained from donors of any age (10–102 years old), and we show that the quality and quantity of DNA, RNA, protein, and fixed lymphocytes obtained do not vary significantly with age. As is frequently observed, the older individuals have higher plasma proportions.