Cajal Body dynamics and association with chromatin are ATP-dependent
Cajal Body dynamics and association with chromatin are ATP-dependent
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DOI：
10.1038/ncb809
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发表时间：
2002-07-01
影响因子：
21.3
通讯作者：
Swedlow, JR
中科院分区：
文献类型：
--
作者：
Platani, M;Goldberg, I;Swedlow, JR
Cajal小体（CB）是一种核细胞器，含有剪接、核糖体生物合成和转录所需的因子。我们之前在活细胞中的分析表明CB是动态结构。在这里，我们表明，CB的流动性所描述的异常扩散和机构之间的交替与染色质和染色质间空间内的扩散协会。CB迁移率增加后ATP耗竭和转录抑制，这表明CB和染色质的协会需要ATP和主动转录。这种行为是从根本上不同的ATP依赖的染色质的流动性观察，并建议一种新的机制管理CB，并可能其他，核体动力学。
Cajal bodies (CBs) are nuclear organelles that contain factors required for splicing, ribosome biogenesis and transcription. Our previous analysis in living cells showed that CBs are dynamic structures. Here, we show that CB mobility is described by anomalous diffusion and that bodies alternate between association with chromatin and diffusion within the interchromatin space. CB mobility increases after ATP depletion and inhibition of transcription, suggesting that the association of CB and chromatin requires ATP and active transcription. This behaviour is fundamentally different from the ATP-dependent mobility observed for chromatin and suggests that a novel mechanism governs CB, and possibly other, nuclear body dynamics.