Morphology and Phylogenetics study on the species of Amphidinium (Gymnodinials, Dinophyceae) from Weizhou Island, Guangxi
Morphology and Phylogenetics study on the species of Amphidinium (Gymnodinials, Dinophyceae) from Weizhou Island, Guangxi
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DOI：
10.11978/2019077
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发表时间：
2020-05-01
期刊：
影响因子：
--
通讯作者：
Lu Songhui
中科院分区：
文献类型：
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作者：
Jiang Lichun;Li Qun;Lu Songhui
本研究采用单细胞毛细管吸管法从广西湄洲岛附近的海藻和珊瑚尸体中分离出前沟藻，并进行了培养。利用光学显微镜、扫描电镜和分子生物学技术对该菌的形态特征进行了鉴定和系统发育分析。结果表明，该海域有3种前沟藻，分别为有盖前沟藻（Amphidiniumoperculatum）、马氏前沟藻（Amphidiniummassertii）和泡状前沟藻（Amphidiniumcaetarea），其中A. operculatum和A.马瑟虫为中国新记录。分析比较了不同种间在形态学和生殖发育上的差异。结果表明，三种前沟藻的形态特征与模式种有显著的一致性。本研究为中国前沟藻属物种的多样性和系统进化提供了新的信息，并将两个前沟藻新种的分布范围扩展到中国南方沿海沃茨。
In this study, strains of Amphidinium from seaweeds and dead corals around Weizhou Island, Guangxi, China were isolated by single cell capillary pipette and cultured. The morphological features and phylogenetic analysis were determined based on light microscopy, scanning electron microscopy and molecular biology techiniques. Three Amphidinium species were identified, namely, Amphidinium operculatum, Amphidinium massertii and Amphidinium caeterae, among which A. operculatum and A. massertii were new records in China. The differences in morphology and phylogeny between different species were analyzed and compared. It was found that the morphological characteristics of the three Amphidinium species were significantly consistent with model species. This study adds information to the diversity and phylogenetic evolution of Amphidinium species in China, and extented the distribution of two new Amphidinium species to the coastal waters of southern China.