Long-range restriction map around 11p13 aniridia locus.
Long-range restriction map around 11p13 aniridia locus.
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11p13 aniridia 基因座周围的长程限制性图谱。
DOI：
10.1007/bf01534921
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Shows,TB
中科院分区：
文献类型：
--
作者：
Davis,LM;Everest,AM;Simola,KO;Shows,TB
使用两个随机DNA标记，和脉冲场凝胶电泳，1.5-Mb的物理地图周围的11 p13无虹膜位点（AN 2）已组装。该图是使用单和双限制性内切酶对正常对照和家族性无虹膜患者的DNA进行组合构建的。无虹膜患者有染色体易位，两个DNA标记位于断裂点两侧。该11 p13断裂点距离DNA标记1104（D11 S95）不超过100 kb，位于断裂点的着丝粒侧。两个CpG岛，相隔550 kb和侧翼的易位，建议上限的基因的大小。
Using two random DNA markers, and pulsed field gel electrophoresis, a 1.5-Mb physical map surrounding the 11p13 aniridia locus(AN2)has been assembled. The map was constructed using a combination of single- and double-restriction digests on DNA from normal controls and a patient transmitting familial aniridia. The aniridia patient has a chromosome translocation and the two DNA markers flank the breakpoint. This 11p13 breakpoint lies no further than 100 kb from the DNA marker 1104(D11S95),located on the centromeric side of the breakpoint. Two CpG islands, separated by 550 kb and flanking the translocation, suggest an upper limit to the size of the gene.