Transcriptomic insights into the effects of abscisic acid on the germination of Magnolia sieboldii K. Koch seed
Transcriptomic insights into the effects of abscisic acid on the germination of Magnolia sieboldii K. Koch seed
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DOI：
10.1016/j.gene.2022.147066
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发表时间：
2022-12-05
期刊：
影响因子：
3.5
通讯作者：
Zhang，Xiao-lin
中科院分区：
文献类型：
--
作者：
Lu，Xiu-jun;Zeng，Wan-qi;Zhang，Xiao-lin
白玉兰。科赫是落叶树种。然而，野生资源还没有得到充分利用。由于过度利用和种子休眠，西伯利亚的产量一直在下降。在我们以前的研究中，玉米种子在自然条件下具有形态生理休眠和较低的萌发率。本研究的目的是确定与休眠维持有关的基因。在本研究中，种子的发芽率。籽粒内源脱落酸(ABA)含量与籽粒内源ABA含量呈负相关。种子萌发的水合作用有三个不同的阶段。确定了5个关键时间点：0小时吸胀(干种子，GZ)，0吸胀后第二天(DAI)，16 DAI，40 DAI，56 DAI。FM的综合成绩单简介。在萌发的五个关键时期，分别用ABA和水处理玉米种子。共鉴定了9641个差异表达基因，其中208个差异表达基因和197个共同差异表达基因分别存在于ABA和水分处理中。与广州相比，SH组在0DAI、16DAI、40DAI和56DAI分别检测到518、696、2133和1535°。ABA组在0、16、40和56天分别检测到666、1725、1560和1415°。在所识别的DEG中，有12722个DEG被标注了GO术语，在ABA处理和SH处理的56DAI的DEG中，前三个丰富的GO术语是不同的。DEGS的KEGG途径富集化分析表明，氧化磷酸化、内质网蛋白质加工、淀粉和蔗糖代谢在种子对ABA的响应中起着重要作用。共获得1926个TFs，并将其划分为72个科。西博尔迪特翻译公司。基因差异表达分析和qRT-PCR结果表明，II期是种子快速成功萌发的关键。本研究首次揭示了ABA调控的INM转录本的整体表达模式。在不同的萌发阶段播种。
Magnolia sieboldiiK. Koch is a deciduous tree species. However, the wild resource ofM. sieboldiihas been declining due to excessive utilization and seed dormancy. In our previous research,M. sieboldiiseeds have morphophysiological dormancy and low germination rates under natural conditions. The aim of the present study was to identify the genes involved in dormancy maintenance. In this study, the germination percentage ofM. sieboldiiseeds negatively correlated with the content of endogenous abscisic acid (ABA). The hydration of seeds for germination showed three distinct phases. Five key time points were identified: 0 h imbibition (dry seed, GZ), 0 day after imbibition (DAI), 16 DAI, 40 DAI, and 56 DAI. The comprehensive transcript profiles ofM. sieboldiiseeds treated with ABA and water at the five key germinating stages were obtained. A total of 9641 differentially expressed genes (DEGs) were identified, and 208 and 197 common DEGs were found throughout the ABA and water treatments, respectively. Compared with that in the GZ, 518, 696, 2133, and 1535 DEGs were identified in the SH group at 0, 16, 40 and 56 DAI, respectively. 666, 1725, 1560 and 1415 DEGs were identified in the ABA group at 0, 16, 40, and 56 DAI, respectively. Among the identified DEGs, 12 722 were annotated with GO terms, the top three enriched GO terms were different among the DEGs at 56 DAI in the ABA vs. SH treatments. KEGG pathway enrichment analysis for DEGs indicated that oxidative phosphorylation, protein processing in endoplasmic reticulum, starch and sucrose metabolism play an important role in seed response to ABA. 1926 TFs are obtained and classified into 72 families from theM. sieboldiitranscriptome. Results of differential gene expression analysis together with qRT-PCR indicated that phase II is crucial for rapid and successful seed germination. This study is the first to present the global expression patterns of ABA-regulated transcripts inM. sieboldiiseeds at different germinating phases.