Muscovy Duck Reovirus Infection Disrupts the Composition of Intestinal Microbiota in Muscovy Ducklings
Muscovy Duck Reovirus Infection Disrupts the Composition of Intestinal Microbiota in Muscovy Ducklings
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DOI：
10.1007/s00284-019-01865-8
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发表时间：
2020-05-01
影响因子：
2.6
通讯作者：
Chen, Shilong
中科院分区：
文献类型：
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作者：
Chen, Xiuqin;Zheng, Min;Chen, Shilong
番鸭呼肠孤病毒（MDRV）对雏番鸭具有高致病性。虽然MDRV感染导致雏鸭急性水样腹泻，但MDRV感染对宿主肠道微生物群组成的影响仍知之甚少。本研究旨在研究MDRV对雏番鸭肠道细菌群落组成的影响。分别用MDRV强毒株MW9710和无菌Hank's液接种3日龄番鸭。使用Illumina MiSeq测序分别在6 dpi和17 dpi分析对照和模拟MDRV感染的雏鸭之间的盲肠微生物群。结果表明，MDRV感染可引起肠粘膜损伤。此外，MDRV感染通过降低微生物丰富度引起肠道微生物群的严重扰动，改变了6 dpi时肠道微生物群某些属的丰富度。MDRV感染组雏鸭产短链脂肪酸细菌（梭菌属、链球菌属和瘤胃球菌属）的相对丰度较对照组降低，而肠杆菌科（邻单胞菌属、埃希氏菌属和变形杆菌属）的相对丰度较对照组增加。此外，微生物群分析显示，由MDRV感染引起的肠道微生物群失调在17 dpi时基本恢复。本研究表明，雏番鸭感染MDRV后肠道菌群发生了变化，主要表现为有益菌的净损失和致病菌的代偿性增殖，可能导致肠黏膜严重病变，最终导致急性腹泻。这些结果将为MDRV感染的病理学提供见解。
Muscovy duck reovirus (MDRV) is highly pathogenic to young Muscovy ducklings. Although MDRV infection results in ducklings' acute watery diarrhea, the effect of MDRV infection on the composition of host's intestinal microbiota remains poorly understood. This study was conducted to investigate the impacts of MDRV on the composition of Muscovy ducklings' intestinal bacterial community. Three-day-old Muscovy ducklings were inoculated with either the virulent MDRV strain MW9710 or sterile Hank's solution, respectively. The cecal microbiota was analyzed between control and mock MDRV-infected ducklings using Illumina MiSeq sequencing at 6 dpi and 17 dpi, respectively. The results indicated that MDRV infection damaged the intestinal mucosa. In addition, MDRV infection caused severe perturbations of gut microbiota by decreasing microbial richness, altering the abundance of certain genera of the gut microbiota at 6 dpi. Specifically, the relative abundance of short chain fatty acids-producing bacteria (including Shuttleworthia, Streptococcus, and Ruminococcus) was reduced in MDRV-infected ducklings than those of control group, whereas, with an enrichment of Enterobacteriaceae (including Plesiomonas, Escherichia_Shigella and Proteus). Furthermore, microbiota analysis showed that the gut microbiota dysbiosis caused by MDRV infection was basically recovered at 17 dpi. Collectively, this study demonstrated that the gut microbiota of Muscovy ducklings were altered due to MDRV infection, mainly featuring as a net loss of beneficial bacteria and a compensatory proliferation of pathogenic bacteria, which may lead to severe pathology to the intestinal mucosa, and ultimately acute diarrhea. These results will provide insights into the pathology of MDRV infection.