Effect of rapamycin on wound healing: An experimental study
Effect of rapamycin on wound healing: An experimental study
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DOI：
10.1016/j.transproceed.2007.04.013
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发表时间：
2007-05-01
影响因子：
0.9
通讯作者：
Haberal, M.
中科院分区：
文献类型：
--
作者：
Ekici, Y.;Emiroglu, R.;Haberal, M.
本研究的目的是研究雷帕霉素（RAPA）对膀胱和腹部伤口愈合的影响。14只雄性Sprague道利大鼠随机接受RAPA（3 mg/d）或安慰剂。进行了中线剖腹手术。切开膀胱并用4-0 Vicryl双层缝合。筋膜用0尼龙缝线缝合，皮肤用表皮下2-0尼龙缝线缝合。平均RAPA水平为9.1 ng/mg。对照组的膀胱、筋膜和真皮中嗜酸性粒细胞和中性粒细胞浸润以及肌成纤维细胞增殖的存在和程度显著较高。各组的淋巴细胞浸润相似。RAPA组的平均微血管密度以及膀胱、筋膜和真皮中表达血管内皮生长因子的细胞百分比显著较低。安慰剂组中筋膜炎性细胞、真皮成纤维细胞和上皮细胞的增殖细胞核抗原标记指数均显著较高。两组间膀胱和筋膜中羟脯氨酸水平无差异。总之，我们发现RAPA治疗通过减少炎性细胞数量、血管生成和肌成纤维细胞增殖来影响伤口愈合过程的所有步骤，因此伤口愈合过程被延迟，因此伤口的拉伸强度降低。
The aim of this study was to investigate the effects of rapamycin (RAPA) on the healing of bladder and abdominal wound closures. Fourteen male Sprague Dawley rats were randomized to receive either RAPA (3 mg/d) or placebo. A midline laparotomy was performed. The bladder was cut and closed with 4-0 Vicryl in a double layer. The fascia was closed with 0 nylon suture, and the skin closed with a subcuticular 2-0 nylon suture. The mean RAPA level was 9.1 ng/mg. Eosinophil and neutrophil infiltration, and the presence and degree of myofibroblast proliferation were significantly higher in the bladder, fascia, and dermis of the control group. Lymphocyte infiltration was similar in each group. Mean microvessel density as well as the percentage of cells expressing vascular endothelial growth factor in the bladder, fascia, and dermis were significantly lower among the RAPA group. Both proliferating cell nuclear antigen labeling indices for inflammatory cells in the fascia, dermal fibroblasts, and epithelial cells in the placebo group were significantly higher. No difference was observed for hydroxyproline levels in both the bladder and fascia between the groups. In conclusion, we found that RAPA treatment affected all steps of the wound healing process by decreasing the inflammatory cell number, angiogenesis, and myofibroblast proliferation, so the wound healing process was delayed and consequently the tensile strength of the wound decreased.