High pathogenicity and low genetic evolution of avian paramyxovirus type I (Newcastle disease virus) isolated from live bird markets in Uganda.
High pathogenicity and low genetic evolution of avian paramyxovirus type I (Newcastle disease virus) isolated from live bird markets in Uganda.
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DOI：
10.1186/1743-422x-11-173
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发表时间：
2014-10-01
期刊：
影响因子：
4.8
通讯作者：
Ducatez MF
中科院分区：
文献类型：
--
作者：
Byarugaba DK;Mugimba KK;Omony JB;Okitwi M;Wanyana A;Otim MO;Kirunda H;Nakavuma JL;Teillaud A;Paul MC;Ducatez MF
纽卡斯尔病仍然是家禽的一种严重疾病，特别是在散养系统中，尽管可以获得交叉保护性疫苗。活禽市场上看起来健康的家禽被怀疑是疾病传播的主要来源，尽管已经进行了有限的研究，以确定市场上是否存在有毒菌株，并了解它们与爆发菌株的关系。本研究评估了从乌干达各地活禽市场的家禽中采集的样本中纽卡斯尔病病毒的发生情况。使用标准方法（平均死亡时间（MDT），脑内致病性指数（ICPI）和融合蛋白切割位点基序的测序）对分离株进行病理分型，并在全融合和血凝素神经氨酸酶基因测序后进行遗传学分析。根据全融合蛋白基因分类系统将分离株分为基因型和亚基因型，并与本地区和世界其他菌株进行比较。在活禽市场的健康家禽中分离到强毒禽副粘病毒I型（APMV-1）（纽卡斯尔病病毒）。这些病毒属于基因型V中的一个新的亚基因型Vd，并与坦桑尼亚和肯尼亚毒株聚在一起。它们的遗传多样性很低。在活禽市场中出现的强毒AMPV-1毒株可能是非商业农场中纽卡斯尔病暴发的来源。
Newcastle disease is still a serious disease of poultry especially in backyard free-range production systems despite the availability of cross protective vaccines. Healthy-looking poultry from live bird markets have been suspected as a major source of disease spread although limited studies have been conducted to ascertain the presence of the virulent strains in the markets and to understand how they are related to outbreak strains. This study evaluated the occurrence of Newcastle disease virus in samples collected from poultry in live bird markets across Uganda. The isolates were pathoyped using standard methods (mean death time (MDT), intracelebral pathogenicity index (ICPI), and sequencing of the fusion protein cleavage site motif) and also phylogenetically analysed after sequencing of the full fusion and hemagglutin-neuraminidase genes. The isolates were classified into genotypes and subgenotypes based on the full fusion protein gene classification system and compared with other strains in the region and world-wide. Virulent avian paramyxovirus type I (APMV-1) (Newcastle disease virus) was isolated in healthy-looking poultry in live bird markets. The viruses belonged to a new subgenotype, Vd, in genotype V, and clustered together with Tanzania and Kenya strains. They harbored low genetic diversity. The occurrence of virulent AMPV-1 strains in live bird markets may serve as sources of Newcastle disease outbreaks in non-commercial farms.