RELATIONSHIP OF HYDROGEN PEROXIDE PRODUCTION BY MYCOPLASMA PULMONIS TO VIRULENCE FOR CATALASEDEFICIENT MICE
RELATIONSHIP OF HYDROGEN PEROXIDE PRODUCTION BY MYCOPLASMA PULMONIS TO VIRULENCE FOR CATALASEDEFICIENT MICE
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DOI：
10.1128/jb.98.3.1036-1040.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
FEINSTEIN, RN
中科院分区：
文献类型：
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作者：
BRENNAN, PC;FEINSTEIN, RN
肺支原体是小鼠肺炎的病原体，每 1010 个菌落形成单位每小时产生约 0.065 微摩尔过氧化氢 (H2O2)。当葡萄糖浓度为0.01m时，H2O2产量增加50%。确定H2O2是否由M产生。肺单胞菌可能与毒力相关，正常、无解酶血症和无解酶小鼠均感染该微生物。感染 M 后三天。肺部疾病 患有肺炎的小鼠明显多于正常小鼠或贫血小鼠。无催化小鼠的肺炎也比其他两组更严重。感染五天后，无反应小鼠的肺炎得到缓解，而正常小鼠则受到严重影响。肺炎的存在和严重程度与支原体的恢复相关。来自病变的肺。过氧化氢酶对 M 影响的体外研究。肺部显示，外源提供的过氧化氢酶刺激了分枝杆菌的生长。 pulmonisat 37°C 并延长其在 25°C 下的存活时间。肺病与红细胞过氧化氢酶活性呈负相关。这些发现表明 H2O2 分泌有助于 M. 的毒力。在没有宿主过氧化氢酶的情况下，肺孢子虫会导致微生物死亡。
Mycoplasma pulmonis, an etiological agent of murine pneumonia, produced about 0.065 μmoles of hydrogen peroxide (H2O2) per hr per 1010colony-forming units. When glucose was present at a concentration of 0.01m, H2O2production was increased by 50%. To determine if H2O2production byM. pulmoniscould be correlated with virulence, normal, acatalasemic, and acatalatic mice were infected with the organism. Three days after infection withM. pulmonissignificantly more acatalatic mice had pneumonia than did normal or acatalasemic mice. The pneumonia in acatalatic mice was also more severe than in the other two groups. Five days after infection, pneumonia in the acatalatic mice was resolved, whereas normal mice were severely affected. The presence of pneumonia and the severity were correlated with the recovery ofM. pulmonisfrom the lesions. In vitro studies of the effect of catalase onM. pulmonisshowed that exogenously supplied catalase stimulated the growth ofM. pulmonisat 37 C and prolonged its survival at 25 C. Hemolysis of sheep blood, guinea pig blood, rabbit blood, and normal and acatalasemic mouse blood byM. pulmoniswas inversely related to the catalase activity of the erythrocytes. These findings suggest that H2O2secretion contributes to the virulence ofM. pulmonisand to the death of the microorganism in the absence of host catalase.