The role of wheat jasmonic acid and ethylene pathways in response to Fusarium graminearum infection
The role of wheat jasmonic acid and ethylene pathways in response to Fusarium graminearum infection
复制标题
小麦茉莉酸和乙烯途径在响应禾谷镰刀菌感染中的作用
DOI：
10.1007/s10725-016-0147-1
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发表时间：
2016
影响因子：
4.2
通讯作者：
Wang Xiue
中科院分区：
文献类型：
--
作者：
Sun Yuxin;Xiao Jin;Jia Xinping;Ke Peibei;He Liqiang;Cao Aizhong;Wang Haiyan;Wu Yufeng;Gao Xiquan;Wang Xiue
为了研究茉莉酸（JA）和乙烯（ET）途径在小麦赤霉病防御中的作用，利用小麦基因芯片技术比较了王水白及其感病突变体NAUH 117 Fhb 1位点基因在赤霉病诱导下穗部的表达。结果表明，绝大多数JA相关基因在望水白中被诱导表达，而NAUH 117中只有少数基因被诱导表达;绝大多数ET相关基因在两种基因型中均表达上调。ELISA检测结果表明，F.禾谷镰刀菌诱导的穗期，王水白内源JA含量增加，而NAUH 117则没有。外源茉莉酸甲酯预处理可减轻小麦病害的严重程度。而外源乙烯利预处理则无此效应。选择JA途径的代表基因--脂转运蛋白基因，利用LTP基因的T-DNA插入突变系在拟南芥系统中进行功能分析。结果表明，突变体的抗赤霉病性比野生型低，证明LTP在拟南芥赤霉病抗性中可能起作用。这些结果表明JA途径在FHB抗性中起着关键作用。
To study the roles of jasmonic acid (JA) and ethylene (ET) pathways in mediating defense against wheatFusariumhead blight (FHB), the expression patterns of genes in theFusarium graminearum-challenged spikes between Wangshuibai and its susceptible mutant NAUH117 at theFhb1locus were compared using wheat microarray. The results showed that most of JA-associated genes were induced in Wangshuibai while only a few were induced in NAUH117, and most ET-associated genes were up-regulated in both genotypes. ELISA assay showed that in theF. graminearum-challenged spikes, endogenous JA content was increased in Wangshuibai while not in NAUH117. Pre-treatment with exogenous methyl JA could decrease the wheat disease severity. However, pretreatment by exogenous ethephon had no such effect. A lipid transfer protein gene, which is a representative gene for JA pathway, was selected for function analysis in Arabidopsis system using a T-DNA insertion mutant line for LTP gene. It was found that the mutant showed compromised FHB resistance compared with its wildtype, proving the possible role of LTP in FHB resistance of Arabidopsis. These results demonstrated that JA pathway should play a critical role in FHB resistance.