Positively charged supported lipid bilayer formation on gold surfaces for neuronal cell culture
Positively charged supported lipid bilayer formation on gold surfaces for neuronal cell culture
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DOI：
10.1116/1.4945306
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发表时间：
2016-06-01
期刊：
影响因子：
2.1
通讯作者：
Offenhaeusser, Andreas
中科院分区：
文献类型：
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作者：
Choi, Sung-Eun;Greben, Kyrylo;Offenhaeusser, Andreas
支撑的脂质双层膜被广泛用作细胞膜模型和传感器平台，但在金表面的使用需要额外的表面修饰或优化的实验条件。在这项工作中，作者表明，如果存在至少30%的带正电荷的脂质，则在生理条件下在等离子体活化的金表面上形成脂质双层，而没有任何其他修饰。从小的单层囊泡的双层形成的细节进行了监测，使用石英晶体微天平在碱性和酸性环境中的耗散。作者还证实，这种带正电荷的双层系统可以维持初级皮质神经元的生长和脂质转移。该方法为在金电极上构建仿生界面提供了一种简便的方法。(C)2016年美国真空学会。
Supported lipid bilayers are widely used as cell membrane models and sensor platforms, but the usage on gold surface needs additional surface modification or optimized experimental conditions. In this work, the authors show lipid bilayer formation on plasma activated gold surfaces in physiological conditions without any other modification if at least 30% positively charged lipids are present. Details of bilayer formation from small unilamellar vesicles were monitored using quartz crystal microbalance with dissipation in both basic and acidic environment. The authors also confirmed that this positively charged bilayer system can sustain primary cortical neuron growth and lipid transfer. This method will provide simple means to construct biomimetic interface on gold electrodes. (C) 2016 American Vacuum Society.