Exclusion of polymeric immunoglobulins and selective immunoglobulin Y transport that recognizes its Fc region in avian ovarian follicles
Exclusion of polymeric immunoglobulins and selective immunoglobulin Y transport that recognizes its Fc region in avian ovarian follicles
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DOI：
10.1016/j.vetimm.2007.10.006
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发表时间：
2008-02-15
影响因子：
1.8
通讯作者：
Murai, Atsushi
中科院分区：
文献类型：
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作者：
Kitaguchi, Kohji;Osada, Kenichi;Murai, Atsushi
在鸟类中，血液免疫球蛋白（IG）Y，相当于哺乳动物的IgG，被选择性地掺入卵泡，但其他类别，伊加和IgM，在卵泡中的丰度要低得多。几种哺乳动物免疫球蛋白，包括IgG和伊加，也纳入卵巢卵泡时，给予鸟类。为了阐明掺入卵泡所需的IG结构，在静脉注射鸡和人Ig后测定了IG摄取。用地高辛标记了3种鸡Ig（cIgY、cIgA和cIgM）和2种人Ig（单体型和多聚型），用ELISA和SDS-PAGE法测定了它们在鹌鹑蛋黄中的摄取。在三种cIg中，cIgY的摄取最高（22%的注射cIgY从蛋黄中回收）。鸡伊加有效地纳入蛋黄时，它形成了一个单体状态。五聚体IgM不能转运到蛋黄中。我们还发现，单体hIgA的摄取比多聚hIgA更有效。这些结果表明，单体形式的保留有助于有效地运输到卵巢卵泡的Ig。另一方面，单体Ig之间的IG摄取仍然不同;例如，时程分析显示，单体cIgY摄取的速率比单体hIgA快约8倍。注射cIgY片段Fc、Fab和F（ab '）2导致Fc片段的最大摄取，与cIgY的摄取水平相同。这些结果表明，存在一个选择性的IgY运输系统，识别其铁区域在禽卵巢卵泡。(c)2007 Elsevier B. V.保留所有权利。
In avian species, blood immunoglobulin (Ig) Y, the equivalent to mammalian IgG, is selectively incorporated into ovarian follicles, but other classes, IgA and IgM, are much less abundant in the follicles. Several mammalian Igs, including IgG and IgA, are also incorporated into ovarian follicles when administered to birds. To clarify the Ig structure required for incorporation into ovarian follicles, Ig uptakes were determined after the intravenous injection of chicken and human Igs. Three chicken Igs (cIgY, cIgA and cIgM) and two human IgAs (monomeric hIgA and polymeric hIgA) were labeled with digoxigenin, and their uptakes into quail (Coturnix japonica) egg yolks were determined by ELISA and SDS-PAGE. The uptake of cIgY was the highest among the three cIgs (22% of injected cIgY was recovered from egg yolks). Chicken IgA was efficiently incorporated into egg yolk when it formed a monomeric state. Pentameric IgM was untransportable into egg yolk. We also found that the uptake of monomeric hIgA was much more efficient than that of polymeric hIgA. These results suggest that the retention of the monomeric form contributes to the efficient transport of Igs into ovarian follicles. On the other hand, Ig uptakes among monomeric Igs nevertheless differed; for example, a time-course analysis showed that the rate of monomeric cIgY uptake was approximately eight times faster than that of monomeric hIgA. The injection of cIgY fragments Fc, Fab and F(ab')2 resulted in the largest uptake of Fc fragment, with the same level as that of cIgY. These results suggest the presence of a selective IgY transport system that recognizes its Fe region in avian ovarian follicles. (c) 2007 Elsevier B.V. All rights reserved.